Dataset Information

Bufffalo serum iTRAQ - Global serum proteomic changes in water buffaloes infected with Fasciola gigantica

ABSTRACT: Fasciola gigantica infects buffalo in Asian and African countries and causes significant economic losses. As a kind of zoonotic parasite disease, human infection has been reported and make fascioliasis patients suffer heavy abdominal pain, anemia and workforce loss. Fasciola can modulate host immune responses to survive from immune attack and some mechanisms have been reported with its antigen protein. Protein, as the chief actors within the cell, presents the dynamic changes of body with its specific changes and serves as unexceptionable biomarkers in many disease-detections. But there is not any study about serum proteomic for diagnostic target seeking in buffalo after infection with F. gigantica. Herein, Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) and Parallel Reaction Monitoring (PRM) technologies were used in the present study to reveal the dynamic changes of proteins in buffalo serum after infected with F. gigantica. Six proteins were found significantly regulated in all three groups which reminds us that they could be used as new diagnostic biomarkers in buffalo throughout F. gigantica infection. All these are new findings in buffalo after infection with F. gigantica and could provide a new insight into the potential possibility in this parasite clearance.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Blood Serum

SUBMITTER: Fu-Kai Zhang

LAB HEAD: Xing-Quan Zhu

PROVIDER: PXD011576 | Pride | 2019-11-12

REPOSITORIES: Pride

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			Action	DRS
	170527_Fusion2_SW_SLQ_B12C1_1_25per_F1_R1.raw	Raw
	170527_Fusion2_SW_SLQ_B12C1_2_25per_F1_R1.raw	Raw
	170527_Fusion2_SW_SLQ_B12C1_3_25per_F1_R1.raw	Raw
	170527_Fusion2_SW_SLQ_B12C1_4_25per_F1_R1.raw	Raw
	170527_Fusion2_SW_SLQ_B12C1_5_25per_F1_R1.raw	Raw

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Global serum proteomic changes in water buffaloes infected with Fasciola gigantica.

Zhang Fu-Kai FK Hu Rui-Si RS Elsheikha Hany M HM Sheng Zhao-An ZA Zhang Wei-Yu WY Zheng Wen-Bin WB Zhu Xing-Quan XQ He Jun-Jun JJ

Parasites & vectors 20190603 1

<h4>Background</h4>The liver fluke Fasciola gigantica modulates several signaling pathways in infected buffaloes to facilitate its survival and establishment of persistent infection. In response to the parasite invasion, buffaloes activate innate and adaptive immune responses to counter the parasite infection. To detect new proteins that might be involved in the interaction between F. gigantica and the buffaloes, and that also might serve as biomarkers for fasciolosis, we used proteomic techniqu ...[more]

PMID: 31159882

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