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TGNap1 proteomic analysis - TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network

ABSTRACT: TGNap1 coimmunoprecipitation from Arabidopsis thaliana.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Whole Body

SUBMITTER: Luciana Renna

LAB HEAD: Federica Brandizzi

PROVIDER: PXD011581 | Pride | 2019-11-08

REPOSITORIES: Pride

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			Action	DRS
	MY_F012357.dat	Other
	qe2014073017.mzML	Mzml
	tempfile (MY_F012357).mzid_tempfile_(MY_F012357).pride.mgf.gz	Mzid
	tempfile (MY_F012357).pride.mztab.gz	Mztab
	tempfileMY_F012357.mzid.gz	Mzid

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Publications

TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network.

Renna Luciana L Stefano Giovanni G Slabaugh Erin E Wormsbaecher Clarissa C Sulpizio Alan A Zienkiewicz Krzysztof K Brandizzi Federica F

Nature communications 20181214 1

Defining convergent and divergent mechanisms underlying the biogenesis and function of endomembrane organelles is fundamentally important in cell biology. In all eukaryotes, the Trans-Golgi Network (TGN) is the hub where the exocytic and endocytic pathways converge. To gain knowledge in the mechanisms underlying TGN biogenesis and function, we characterized TGNap1, a protein encoded by a plant gene of unknown function conserved with metazoans. We demonstrate that TGNap1 is a TGN protein required ...[more]

PMID: 30552321

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TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network.

Project description:Defining convergent and divergent mechanisms underlying the biogenesis and function of endomembrane organelles is fundamentally important in cell biology. In all eukaryotes, the Trans-Golgi Network (TGN) is the hub where the exocytic and endocytic pathways converge. To gain knowledge in the mechanisms underlying TGN biogenesis and function, we characterized TGNap1, a protein encoded by a plant gene of unknown function conserved with metazoans. We demonstrate that TGNap1 is a TGN protein required for the homeostasis of biosynthetic and endocytic traffic pathways. We also show that TGNap1 binds Rab6, YIP4 and microtubules. Finally, we establish that TGNap1 contributes to microtubule-dependent biogenesis, tracking and function of a TGN subset, likely through interaction with Rab6 and YIP4. Our results identify an important trafficking determinant at the plant TGN and reveal an unexpected reliance of post-Golgi traffic homeostasis and organelle biogenesis on microtubules in plants.

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Sphingomyelin homeostasis is required to form functional enzymatic domains at the trans-Golgi network.

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Regulation of microtubule-dependent recycling at the trans-Golgi network by Rab6A and Rab6A'.

Project description:The small GTPase rab6A but not the isoform rab6A' has previously been identified as a regulator of the COPI-independent recycling route that carries Golgi-resident proteins and certain toxins from the Golgi to the endoplasmic reticulum (ER). The isoform rab6A' has been implicated in Golgi-to-endosomal recycling. Because rab6A but not A', binds rabkinesin6, this motor protein is proposed to mediate COPI-independent recycling. We show here that both rab6A and rab6A' GTP-restricted mutants promote, with similar efficiency, a microtubule-dependent recycling of Golgi resident glycosylation enzymes upon overexpression. Moreover, we used small interfering RNA mediated down-regulation of rab6A and A' expression and found that reduced levels of rab6 perturbs organization of the Golgi apparatus and delays Golgi-to-ER recycling. Rab6-directed Golgi-to-ER recycling seems to require functional dynactin, as overexpression of p50/dynamitin, or a C-terminal fragment of Bicaudal-D, both known to interact with dynactin inhibit recycling. We further present evidence that rab6-mediated recycling seems to be initiated from the trans-Golgi network. Together, this suggests that a recycling pathway operates at the level of the trans-Golgi linking directly to the ER. This pathway would be the preferred route for both toxins and resident Golgi proteins.

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Kinesin adapter JLP links PIKfyve to microtubule-based endosome-to-trans-Golgi network traffic of furin.

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Cab45 is required for Ca(2+)-dependent secretory cargo sorting at the trans-Golgi network.

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