Dataset Information

Genome-wide postnatal changes in immunity following fetal inflammatory response

ABSTRACT: The fetal inflammatory response (FIR) increases the risk of perinatal brain injury, particularly in extremely low gestational age newborns (ELGANs, < 28 weeks of gestation). One of the mechanisms contributing to such a risk is a postnatal intermittent or sustained systemic inflammation (ISSI) following FIR. The link between prenatal and postnatal systemic inflammation is supported by the presence of well-established inflammatory biomarkers in the umbilical cord and peripheral blood. However, the extent of molecular changes contributing to this association is unknown. Using RNA sequencing and mass spectrometry proteomics, we profiled the transcriptome and proteome of archived neonatal dried blood spot (DBS) specimens from 21 ELGANs. Comparing FIR-affected and unaffected ELGANs, we identified 782 gene and 27 protein expression changes of 50% magnitude or more, and an experiment-wide significance level below 5% false discovery rate. These expression changes confirm the robust postnatal activation of the innate immune system in FIR-affected ELGANs and reveal for the first time an impairment of their adaptive immunity. In turn, the altered pathway provide clues about the molecular mechanisms triggering ISSI after FIR, and the onset of perinatal brain injury.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Blood

SUBMITTER: Eduard Sabidó

LAB HEAD: Eduard Sabidó

PROVIDER: PXD011626 | Pride | 2019-06-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	180506_S_ROCA_01_01_BS03.raw	Raw
	180506_S_ROCA_03_01_BS05.raw	Raw
	180506_S_ROCA_04_01_BS06.raw	Raw
	180506_S_ROCA_05_01_BS07.raw	Raw
	180506_S_ROCA_06_01_BS08.raw	Raw

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Genome-wide postnatal changes in immunity following fetal inflammatory response.

Costa Daniel D Bonet Núria N Solé Amanda A González de Aledo-Castillo José Manuel JM Sabidó Eduard E Casals Ferran F Rovira Carlota C Nadal Alfons A Marin Jose Luis JL Cobo Teresa T Castelo Robert R

The FEBS journal 20201024 7

The fetal inflammatory response (FIR) increases the risk of perinatal brain injury, particularly in extremely low gestational age newborns (ELGANs, < 28 weeks of gestation). One of the mechanisms contributing to such a risk is a postnatal intermittent or sustained systemic inflammation (ISSI) following FIR. The link between prenatal and postnatal systemic inflammation is supported by the presence of well-established inflammatory biomarkers in the umbilical cord and peripheral blood. However, the ...[more]

PMID: 33006196

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