Dataset Information

Interactive Peptide Spectral Annotator Experimental Data

ABSTRACT: Abstract Here we present IPSA, an innovative web-based spectrum annotator that visualizes and characterizes peptide tandem mass spectra. A tool for the scientific community, IPSA can visualize peptides collected using a wide variety of experimental and instrumental configurations. Annotated spectra are customizable via a selection of interactive features and can be exported as editable scalable vector graphics to aid in the production of publication-quality figures. Single spectra can be analyzed through provided forms, while multiple peptide spectral matches can be uploaded directly to the server as CSV, MGF, or mzML files. IPSA facilitates the characterization of experimental MS/MS performance through the optional export of fragment ion statistics from one to many peptide spectral matches. This resource is made freely accessible at http://interactivepeptidespectralannotator.com, and the source code is available for inspection at https://github.com/dbrademan/IPSA-dev for custom implementations. This repository contains the raw data, sequence databases, and peptide identifications utilized in the manuscript.

INSTRUMENT(S): LTQ Orbitrap Elite, Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Dain Brademan

LAB HEAD: Joshua J. Coon

PROVIDER: PXD011695 | Pride | 2019-05-15

REPOSITORIES: pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	010090_MusMusculus_Uniprot_ISOFORMS_CONCAT_20161109.fasta	Fasta
	559292_SaccharomycesCerevisiae_Uniprot_ISOFORMS_CONCAT_20180626.fasta	Fasta
	dbrademan_Yeast_HCD.raw	Raw
	dbrademan_Yeast_HCD_peptides.csv	Csv
	nriley_Mouse_AI-ETD.raw	Raw

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Publications

Interactive Peptide Spectral Annotator: A Versatile Web-based Tool for Proteomic Applications.

Brademan Dain R DR Riley Nicholas M NM Kwiecien Nicholas W NW Coon Joshua J JJ

Molecular & cellular proteomics : MCP 20190514 8 suppl 1

Here we present IPSA, an innovative web-based spectrum annotator that visualizes and characterizes peptide tandem mass spectra. A tool for the scientific community, IPSA can visualize peptides collected using a wide variety of experimental and instrumental configurations. Annotated spectra are customizable via a selection of interactive features and can be exported as editable scalable vector graphics to aid in the production of publication-quality figures. Single spectra can be analyzed through ...[more]

PMID: 31088857

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