Dataset Information

Fumarate effect on the Escherichia coli proteome at aerobic and anaerobic growth conditions

ABSTRACT: The changes in protein composition of E. coli were studied in a global proteomic approach for aerobic growth without or with fumarate as carbon source (glycerol + O2, fumarate + O2, respectively) and anaerobic growth without or with fumarate as carbon source (glycerol + DMSO, glycerol + DMSO + fumarate, respectively). The experiments should unravel the changes in response to fumarate under aerobic (i) and anaerobic (ii) conditions, and more generally (iii) the fumarate proteome under aerobic versus anaerobic conditions. Fumarate was used as the C4DC due to its capability to support aerobic and anaerobic growth, and glycerol was used as the alternative carbon source that exerts no or only weak carbon catabolite repression.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Escherichia Coli

SUBMITTER: Elke Hammer

LAB HEAD: Elke Hammer

PROVIDER: PXD011760 | Pride | 2019-11-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	170413_S1_DI_EH_DcuB_W3110_0mMFumarat_BR1_M_01.7z	Other
	170413_S1_DI_EH_DcuB_W3110_0mMFumarat_BR1_M_01.mzML	Mzml
	170413_S1_DI_EH_DcuB_W3110_0mMFumarat_BR2_M_08.7z	Other
	170413_S1_DI_EH_DcuB_W3110_0mMFumarat_BR2_M_08.mzML	Mzml
	170413_S1_DI_EH_DcuB_W3110_0mMFumarat_BR3_M_09.7z	Other

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Publications

Fumarate dependent protein composition under aerobic and anaerobic growth conditions in Escherichia coli.

Surmann Kristin K Stopp Marius M Wörner Sebastian S Dhople Vishnu M VM Völker Uwe U Unden Gottfried G Hammer Elke E

Journal of proteomics 20191114

In the absence of sugars, C4-dicarboxylates (C4DC) like fumarate represent important substrates for growth of Escherichia coli. Aerobically, C4DCs are oxidized to CO<sub>2</sub> whereas anaerobically, C4DCs are used for fumarate respiration. In order to determine the impact of fumarate under aerobic and anaerobic conditions, proteomes of E. coli W3110 grown aerobically or anaerobically with fumarate and/or the non-C4DC substrate glycerol were comparatively profiled by nanoLC-MS/MS. Membrane enri ...[more]

PMID: 31734389

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