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Analysis of endogenous peptides released from osteoarthritic articular cartilage unravels novel pathogenic markers

ABSTRACT: We have performed a peptidomic strategy to identify endogenous peptides (neopeptides) that are released from knee and/or hip OA cartilage, which may serve as disease markers.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Articular Cartilage

DISEASE(S): Osteoarthritis

SUBMITTER: valentina calamia

LAB HEAD: Francisco J Blanco

PROVIDER: PXD011800 | Pride | 2019-07-30

REPOSITORIES: Pride

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			Action	DRS
	N1.mgf	Mgf
	N1.msf	Msf
	N1.raw	Raw
	N2.mgf	Mgf
	N2.msf	Msf

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Publications

Analysis of Endogenous Peptides Released from Osteoarthritic Cartilage Unravels Novel Pathogenic Markers.

Fernández-Puente Patricia P González-Rodríguez Lucía L Calamia Valentina V Picchi Florencia F Lourido Lucía L Camacho-Encina María M Oreiro Natividad N Rocha Beatriz B Paz-González Rocío R Marina Anabel A García Carlos C Blanco Francisco J FJ Ruiz-Romero Cristina C

Molecular & cellular proteomics : MCP 20190727 10

Osteoarthritis (OA) is a pathology characterized by the loss of articular cartilage. In this study, we performed a peptidomic strategy to identify endogenous peptides (neopeptides) that are released from human osteoarthritic tissue, which may serve as disease markers. With this aim, secretomes of osteoarthritic and healthy articular cartilages obtained from knee and hip were analyzed by shotgun peptidomics. This discovery step led to the identification of 1175 different peptides, corresponding t ...[more]

PMID: 31352363

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Project description:Developmental dysplasia of the hip (DDH) is one of the significant risk factors for hip osteoarthritis. In order to investigate the factors that induce early articular cartilage degeneration of the hip joints that are exposed to reduced dynamic loads arising from hip dislocation , we created rodent models of hip dislocation by swaddling. Notably, expression of periostin (Postn) was increased in the acetabular articular cartilage of the DDH models; Postn was a candidate gene associated with early articular cartilage degeneration. We showed that early articular cartilage degeneration was suppressed in Postn-/- DDH mice. Furthermore, a microgravity environment induced the expression of Postn in chondrocytes through STAT3 signaling. Postn induced catabolic factors, interleukin-6 and matrix metalloproteinase 3, in articular chondrocytes through integrin-nuclear factor κB signaling. Additionally, interleukin-6 stimulated Postn expression through STAT3 signaling. Thus, Postn plays a critical role in early articular cartilage degeneration associated with hip dislocation.

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