Dataset Information

Protein phosphorylation landscape of mouse spermatids during spermiogenesis

ABSTRACT: Tadpole-shaped sperm is formed from round spermatid by spermiogenesis, a process with dramatic morphological changes in the final stage of spermatogenesis in testis. Protein phosphorylation, as one of the most important post-translational modifications, can regulate spermiogenesis, however, there is a lack of systemic analysis for phosphorylation events in this process. By large-scale phosphoproteome profiling using IMAC and TiO2 enrichment, we identified 18,980 phosphorylation sites in 4,628 phosphoproteins in mouse purified spermatids undergoing spermiogenesis. The identified phosphoproteins were significantly enriched in RNA transport, cell-cell adhesion, centrosome, microtubule and cilliogenesis. Further characterization of the kinase substrate relationship network demonstrated enrichment of phosphorylation substrates were related to the regulation of spermiogenesis. This global protein phosphorylation landscape of spermiogenesis showed wide phosphoregulation of diverse processes during spermiogenesis and could help further characterization of the process of sperm generation.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis

SUBMITTER: hao zhang

LAB HEAD: Guo, Xuejiang

PROVIDER: PXD011890 | Pride | 2019-03-29

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	mmSpermatid-rep1-frac1-phospho-enriched-IMAC-20170503.raw	Raw
	mmSpermatid-rep1-frac1-phospho-enriched-TiO2-20170509.raw	Raw
	mmSpermatid-rep1-frac10-phospho-enriched-IMAC-20170518.raw	Raw
	mmSpermatid-rep1-frac10-phospho-enriched-TiO2-20170522.raw	Raw
	mmSpermatid-rep1-frac11-phospho-enriched-IMAC-20170513.raw	Raw

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Publications

The Protein Phosphorylation Landscape of Mouse Spermatids during Spermiogenesis.

Li Yan Y Cheng Yiwei Y Zhu Tianyu T Zhang Hao H Li Wen W Guo Yueshuai Y Qi Yaling Y Chen Xu X Zhang Jun J Sha Jiahao J Zhou Zuomin Z Zhu Hui H Guo Xuejiang X

Proteomics 20190508 11

The characteristic tadpole shape of sperm is formed from round spermatids via spermiogenesis, a process which results in dramatic morphological changes in the final stage of spermatogenesis in the testis. Protein phosphorylation, as one of the most important post-translational modifications, can regulate spermiogenesis; however, the phosphorylation events taking place during this process have not been systematically analyzed. In order to better understand the role of phosphorylation in spermioge ...[more]

PMID: 30901149

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