Dataset Information

Post-column solvent addition enhances sensitivity of microflow-based bottom-up proteomics

ABSTRACT: We demonstrate that post-column addition of organic solvents leads to a strong enhancement of electrospray stability in microflow LC-MS experiments improving the sensitivity across the entire gradient, for early eluting peptides by up to tenfold. Post-column solvent addition did not negatively affect chromatographic performance and resulted in an overall 28%-36% increase in identifications at both protein- and peptide-level. Post-column solvent addition can be easily adopted on any microflow LC-MS/MS system.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Mateusz Lacki

LAB HEAD: Stefan Tenzer

PROVIDER: PXD011970 | Pride | 2019-05-28

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2018-04_MF_PC_vs_noPC_userdesigned20181024-155736_peptide_quantification_report.xlsx	Xlsx
	2018-04_MF_PC_vs_noPC_userdesigned20181024-155736_quantification_report.xlsx	Xlsx
	2018-04_Microflow_final_runs_135_25_750_120min_251_peptide_quantification_report.xlsx	Xlsx
	2018-04_Microflow_final_runs_135_25_750_120min_251_quantification_report.xlsx	Xlsx
	2018-04_Microflow_final_runs_135_25_750_35min_0_5ug_251_peptide_quantification_report.xlsx	Xlsx

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Publications

Enhancing Sensitivity of Microflow-Based Bottom-Up Proteomics through Postcolumn Solvent Addition.

Distler Ute U Łącki Mateusz Krzysztof MK Schumann Sven S Wanninger Markus M Tenzer Stefan S

Analytical chemistry 20190604 12

The introduction of more sensitive mass spectrometers allows researchers to adapt front-end liquid chromatography (LC) to individual needs for the analysis of complex proteomes. Where absolute sensitivity is not paramount, it is advantageous to switch from a highly sensitive nanoflow-LC setup, the de facto standard platform in mass-spectrometry (MS)-based discovery proteomics, to a more robust, high-throughput-compatible microflow or conventional-flow setup. To enhance the microflow-LC-MS electr ...[more]

PMID: 31117400

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