Proteomics

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A Novel Nutrient Deprivation-Induced Neonicotinoid Insecticide Acetamiprid Degradation by Ensifer adhaerens CGMCC 6315


ABSTRACT: Proteomic Analysis. The proteomic expression of CGMCC 6315 under different nutrient concentration conditions was investigated by isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics. The CGMCC 6315 was cultured in LB broth and 1/15LB broth as described above, after which the strains were collected by centrifugation at 10,000× g for 10 min at 4°C. Protein extraction, digestion, iTRAQ labeling and peptide fractionation were performed using the protocol described by Jin et al. 29. Protein identification was conducted using a LC-20AD nano-HPLC instrument (Shimadzu, Kyoto, Japan) equipped with a Q EXACTIVE tandem mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) for data-dependent acquisition detection by nano-electrospray ionization. The raw MS/MS data were converted into MGF format by the thermo scientific tool Proteome Discoverer, and the exported MGF files were searched using Mascot (version 2.3.02) against the selected database containing 7546 CGMCC 6315 coding genes. The IQuant software was used for quantitative analysis of the labeled peptides with isobaric tags. Fold changes of >1.7 with p-values <0.05 were used as a cut off for differentially regulated proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Ensifer Adhaerens

TISSUE(S): Cell Culture

SUBMITTER: sun Shi-Lei  

LAB HEAD: Shi-Lei Sun

PROVIDER: PXD012014 | Pride | 2018-12-12

REPOSITORIES: Pride

Dataset's files

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Action DRS
F18FTSECKF2034_I-YwVDj003_rep1.dat Other
P180397_1.raw Raw
P180397_10.raw Raw
P180397_11.raw Raw
P180397_12.raw Raw
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Publications

A Novel Nutrient Deprivation-Induced Neonicotinoid Insecticide Acetamiprid Degradation by Ensifer adhaerens CGMCC 6315.

Sun Shilei S   Fan Zhixia Z   Zhao Yunxiu Y   Guo Leilei L   Dai Yijun Y  

Journal of agricultural and food chemistry 20181221 1


Biodegradation of pesticide pollution is often restricted by environmental pressures, such as nutrient deprivation. Ensifer adhaerens CGMCC 6315 could overcome this issue and degrade neonicotinoid acetamiprid (ACE) efficiently under low nutrient stimuli. The ACE degradation rate improved by 33.1-fold when the lysogeny broth content for cell culture was decreased to 1/15-fold. Resting cells of CGMCC 6315 degraded 94.4% of 200 mg/L ACE in 12 h and quickly eliminated 87.8% of 5 mg/kg of residual so  ...[more]

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