Proteomics

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Linking the Vegetative Cell and Spore Proteomes of Peptoclostridium difficile


ABSTRACT: Peptoclostridium difficile, an anaerobic pathogen, known as causative agent of pseudomembranous colitis and nosocomial diarrhoea. It can also form highly resistant endospores which lay the basic foundations of infection prior germination in the human guts. P. difficile exploits toxins TcdA and TcdB as well as a plethora of other proteins to infect the host. The present study is exclusively focused on the interconnection between vegetative cell and spore proteomes. A novel method developed for 15N metabolic labelling of P. difficile vegetative cells has enabled mass spectrometric quantification of relative protein levels of this pathogen. A total of 1095 proteins has been identified over the combined spores and vegetative cells. From this 796 proteins have been relatively and reproducibly quantified between spores and vegetative cells. Of the quantified proteins 80% are common to both the vegetative cells and spores, indicating that pathogenic P. difficile employs a relatively modest proteomic changeover for survival. Also of the identified proteins are 20%putative membrane proteins which may provide essential targets to combat P. difficile infections. Detailed data analysis has qualified potential biomarkers for diagnostic purposes.

INSTRUMENT(S): apex ultra

ORGANISM(S): Peptoclostridium Difficile (strain 630) (clostridium Difficile)

TISSUE(S): Prokaryotic Cell, Endospore

SUBMITTER: Wishwas R. Abhyankar  

LAB HEAD: Chris G. de Koster

PROVIDER: PXD012030 | Pride | 2020-05-26

REPOSITORIES: Pride

Dataset's files

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Action DRS
CW3066_analysis.baf Other
CW3067_analysis.baf Other
CW3068_analysis.baf Other
CW3069_analysis.baf Other
CW3070_analysis.baf Other
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Publications

Vegetative Cell and Spore Proteomes of <i>Clostridioides difficile</i> Show Finite Differences and Reveal Potential Protein Markers.

Abhyankar Wishwas R WR   Zheng Linli L   Brul Stanley S   de Koster Chris G CG   de Koning Leo J LJ  

Journal of proteome research 20191014 11


<i>Clostridioides difficile</i>-associated infection (CDI) is a health-care-associated infection caused, as the name suggests, by obligate anaerobic pathogen <i>C. difficile</i> and thus mainly transmitted via highly resistant endospores from one person to the other. In vivo, the spores need to germinate into cells prior to establishing an infection. Bile acids and glycine, both available in sufficient amounts inside the human host intestinal tract, serve as efficient germinants for the spores.  ...[more]

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