Proteomics

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Linking the Vegetative Cell and Spore Proteomes of Peptoclostridium difficile


ABSTRACT: Peptoclostridium difficile, an anaerobic pathogen, known as causative agent of pseudomembranous colitis and nosocomial diarrhoea. It can also form highly resistant endospores which lay the basic foundations of infection prior germination in the human guts. P. difficile exploits toxins TcdA and TcdB as well as a plethora of other proteins to infect the host. The present study is exclusively focused on the interconnection between vegetative cell and spore proteomes. A novel method developed for 15N metabolic labelling of P. difficile vegetative cells has enabled mass spectrometric quantification of relative protein levels of this pathogen. A total of 1095 proteins has been identified over the combined spores and vegetative cells. From this 796 proteins have been relatively and reproducibly quantified between spores and vegetative cells. Of the quantified proteins 80% are common to both the vegetative cells and spores, indicating that pathogenic P. difficile employs a relatively modest proteomic changeover for survival. Also of the identified proteins are 20%putative membrane proteins which may provide essential targets to combat P. difficile infections. Detailed data analysis has qualified potential biomarkers for diagnostic purposes.

INSTRUMENT(S): apex ultra

ORGANISM(S): Peptoclostridium Difficile (strain 630) (clostridium Difficile)

TISSUE(S): Prokaryotic Cell, Endospore

SUBMITTER: Wishwas R. Abhyankar  

LAB HEAD: Chris G. de Koster

PROVIDER: PXD012030 | Pride | 2020-05-26

REPOSITORIES: Pride

Dataset's files

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Action DRS
CW3066_analysis.baf Other
CW3067_analysis.baf Other
CW3068_analysis.baf Other
CW3069_analysis.baf Other
CW3070_analysis.baf Other
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Publications

Assessments of Water-Soluble Inorganic Ions and Heavy Metals in Atmospheric Dustfall and Topsoil in Lanzhou, China.

Li Yingquan Y   Zhao Baowei B   Duan Kaixiang K   Cai Juexian J   Niu Wujiang W   Dong Xiao X  

International journal of environmental research and public health 20200424 8


The chemical features of atmospheric dustfall and topsoil in the same region could reflect the processes of the migration, transport, and diffusion of pollutants in the atmospheric-soil system. Samples of atmospheric dustfall and topsoil were collected in Lanzhou City. The contents and correlation of water-soluble inorganic ions (WSIIs) and heavy metals in dustfall and topsoil were analyzed, the sources of heavy metals and WSIIs in dustfall were distinguished, and the potential ecological risks  ...[more]

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