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TEAD1-DBD-DNA interaction studied by structural MS

ABSTRACT: TEAD transcription factors mediate gene expression regulation through interactions with their DNA response M-CAT motif. They are active mainly during growth and development and induce gene expression of proteins involved in cell proliferation, differentiation or apoptosis prevention. TEADs and many of their target proteins are known to be upregulated in several types of cancers. To study the interaction of DNA binding domain of TEAD1 with M-CAT motifs of different orientations originating from regulatory regions of human genes (CTGF, SRF, C-MYC and GLUT1), dissociation constant of each complex was determined using fluorescence anisotropy assay. Structural mass spectrometry techniques (H/D exchange and quantitative chemical cross-linking) and molecular docking were utilized for structural characterization of the binding interface. In-vitro results were correlated with situation in living cells studied by ChIP and Q-PCR.

INSTRUMENT(S): Bruker Daltonics solarix series

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Jurkat Cell

SUBMITTER: Ruzena Filandrova

LAB HEAD: Petr Novak

PROVIDER: PXD012127 | Pride | 2020-12-08

REPOSITORIES: Pride

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			Action	DRS
	ProteinReport-TEAD-DBD_HDXmapping_2019-07-0413-00-12-1407895202979206991.pdf	Pdf
	SearchParameterReport-Batch-TEAD-DBD_2017-10-0223-27-42-8436782128958010671.pdf	Pdf
	TEAD-CMYCen_10m_1_11829.mzML	Mzml
	TEAD-CMYCen_10m_2_11830.mzML	Mzml
	TEAD-CMYCen_10m_3_11831.mzML	Mzml

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