Dataset Information

Succinylome analysis reveals the involvement of lysine succinylation in extreme resistance of Deinococcus radiodurans

ABSTRACT: Increasing evidences showing that succinylation of lysine residue mainly regulates enzymes involved in the carbon metabolism pathway, both in prokaryotic and eukaryotic cells. Deinococcus radiodurans is one of the most radioresistant organisms on earth, which is famous for its robust resistance. A major goal in the current study of protein succinylation is to explore its function in D. radiodurans. We used high-resolution liquid chromatography-mass spectrometry (LC-MS/MS) for qualitative proteomics to perform a global succinylation analysis of D. radiodurans and identified 492 succinylation sites on 270 proteins. These identified proteins are involved in a variety of biological process and pathways.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Deinococcus Radiodurans

TISSUE(S): Whole Body

SUBMITTER: Congli Zhou

LAB HEAD: Ye Zhao

PROVIDER: PXD012149 | Pride | 2021-04-26

REPOSITORIES: Pride

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Succinylome Analysis Reveals the Involvement of Lysine Succinylation in the Extreme Resistance of Deinococcus radiodurans.

Zhou Congli C Dai Jingli J Lu Huizhi H Chen Zijing Z Guo Miao M He Yuan Y Gao Kaixuan K Ge Tong T Jin Jiayu J Wang Liangyan L Tian Bing B Hua Yuejin Y Zhao Ye Y

Proteomics 20190925 20

Increasing evidence shows that the succinylation of lysine residues mainly regulates enzymes involved in the carbon metabolism pathway, in both prokaryotic and eukaryotic cells. Deinococcus radiodurans is one of the most radioresistant organisms on earth and is famous for its robust resistance. A major goal in the current study of protein succinylation is to explore its function in D. radiodurans. High-resolution LC-MS/MS is used for qualitative proteomics to perform a global succinylation analy ...[more]

PMID: 31487437

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Project description:Abstract Background The extraordinarily resistant bacterium Deinococcus radiodurans withstands harsh environmental conditions present in outer space. Deinococcus radiodurans was exposed for one year outside the International Space Station within Tanpopo orbital mission to investigate microbial survival and space travel. In addition, a ground-based simulation experiment with conditions, mirroring those from Low Earth orbit, was performed. Methods We monitored Deinococcus radiodurans cells during early stage of recovery after Low Earth orbit exposure using electron microscopy tools. Furthermore, proteomic, transcriptomic and metabolomic analyses were performed to identify molecular mechanisms responsible for the survival of Deinococcus radiodurans in Low Earth orbit. Results D. radiodurans cells exposed to low Earth orbit conditions do not exhibit any morphological damage. However, an accumulation of numerous outer-membrane associated vesicles was observed. On levels of proteins and transcripts, a multifaceted response was detected to alleviate cell stress. The UvrABC endonuclease excision repair mechanism was triggered to cope with DNA damage. Defense against reactive oxygen species is mirrored by the increased abundance of catalases and is accompanied by the increased abundance of putrescine which works as scavenging molecule. In addition, several proteins and mRNAs, responsible for regulatory and transporting functions showed increased abundances. The decrease in primary metabolites indicate alternations in the energy status, which is needed to repair damaged molecules. Conclusion Low Earth orbit induced molecular rearrangements trigger multiple components of metabolic stress response and regulatory networks in exposed microbial cells. Presented results show that the non-sporulating bacterium Deinococcus radiodurans survived long-term Low Earth orbit exposure if wavelength below 200 nm are not present, which mirrors the UV spectrum of Mars, where CO2 effectively provides a shield below 190 nm. These results should be considered in the context of planetary protection concerns and the development of new sterilization techniques for future space missions.

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