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Equine energy-storing SDFT tendon development, Proteomic analysis of IFM and fascicles through development

ABSTRACT: Equine energy-storing SDFT tendon development, Proteomic analysis of IFM and fascicles through development

INSTRUMENT(S): Q Exactive

ORGANISM(S): Equus Caballus (horse)

TISSUE(S): Tendon

SUBMITTER: Danae Zamboulis

LAB HEAD: Peter Clegg

PROVIDER: PXD012169 | Pride | 2021-09-08

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	DS_E1_FM_foetus_1h.mgf	Mgf
	DS_E1_FM_foetus_1h.mzid.gz	Mzid
	DS_E1_FM_foetus_1h.pride.mgf.gz	Mgf
	DS_E1_FM_foetus_1h.pride.mztab.gz	Mztab
	DS_E1_FM_foetus_1h.raw	Raw

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Publications

Postnatal mechanical loading drives adaptation of tissues primarily through modulation of the non-collagenous matrix.

Zamboulis Danae E DE Thorpe Chavaunne T CT Ashraf Kharaz Yalda Y Birch Helen L HL Screen Hazel Rc HR Clegg Peter D PD

eLife 20201016

Mature connective tissues demonstrate highly specialised properties, remarkably adapted to meet their functional requirements. Tissue adaptation to environmental cues can occur throughout life and poor adaptation commonly results in injury. However, the temporal nature and drivers of functional adaptation remain undefined. Here, we explore functional adaptation and specialisation of mechanically loaded tissues using tendon; a simple aligned biological composite, in which the collagen (fascicle) ...[more]

PMID: 33063662

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Structure-function Relationships in Human Tendons: Interfascicular Matrix Specialisation in the Achilles Tendon C4PR_LIV

Project description:Tendon is a highly aligned connective tissue, in which the macro-structure consists of collagen-rich fascicles surrounded by interfascicular matrix (IFM). In a series of recent studies in equine tissue, we have demonstrated specialisation of tendon composition, structure and mechanics to achieve the tendon’s functional requirements, specifically reporting extensive specialisation of the IFM region in the energy storing superficial digital flexor tendon. We have also demonstrated loss of functional specialisms with ageing, leading to a hypothesised new paradigm for tendinopathy, focused on the importance of the IFM. However, to date, there have been no studies focused on structure-function specialisation or the IFM in functionally distinct human tendons. Here, we compare the positional anterior tibialis tendon and energy storing Achilles tendon, performing a detailed analysis of the composition and mechanical properties of both fascicle and IFM regions, to test the hypothesis that the IFM in the energy storing Achilles tendon has specialised composition and mechanical properties, and that these specialisations are lost with ageing. We demonstrate that the IFM is specialised in the energy storing Achilles tendon, with greater elasticity and fatigue resistance than in the positional anterior tibialis tendon. While there were few age-related alterations in mechanics, we did identify age-related alterations in the IFM proteome of the Achilles tendon specifically, which is predicted to be regulated by TGF-beta signalling and may be responsible for the trend towards decreased fatigue resistance observed in the Achilles IFM with ageing.

2020-11-11 | PXD018212 | Pride

Equine tendon LC-MSMS, C4PR_LIV

Project description:the aim of this study was to isolate, characterise and compare the proteome of the IFM and fascicular matrix (FM) in the equine superficial digital flexor tendon (SDFT). We hypothesised that the proteomic profile would differ between the FM and the IFM, with greater complexity and faster turnover in the IFM. Regions of the SDFT were isolated using laser capture microdissection and the protein profile assessed using mass spectrometry. 246 proteins were identified in the IFM, and 141 were identified in the FM. 60 proteins showed a 2-fold difference in abundance between the IFM and FM, including greater abundance of collagen-III and cellular proteins in the IFM, and higher levels of collagens-I and XII, and fibromodulin in the FM. A greater number of neopeptides were identified in the IFM, indicating more rapid turnover than in the FM. There were few alterations in protein abundance with ageing, but the number of neopeptides decreased with age in the IFM specifically, suggesting a decrease in IFM turnover in aged individuals. These results support the hypothesis, showing distinct protein profiles and indicating more rapid turnover in the IFM than in the FM, which appears to decline with ageing in the IFM specifically. This may result in alterations in IFM mechanical properties in aged tendon, contributing to age-related tendon injury.

2016-02-08 | PXD002979 | Pride

Interleukin-1b in Tendon Injury Enhances Reparative Gene and Protein Expression in Mesenchymal Stem Cells

Project description:Intralesional mesenchymal stem cell (MSC) therapy has improved tissue architecture and reinjury rates in equine tendon injury; however, the mechanisms by which they promote repair are still being investigated. Therefore, the objectives of this study were to determine how the predominate pro-inflammatory cytokines present in a surgically induced model of equine tendon injury modulate MSC gene and protein expression.

2022-07-26 | GSE208569 | GEO

Indirect Flight Muscle (IFM) expression profiling of actin and myosin nulls in Drosophila melanogaster

Project description:Expression profiling of IFMs from 1-2 day old adult male flies of 3 genotypes: Canton-S, IFM-specific actin null (Act88FKM88) and IFM-specific myosin null (Mhc7). Results provide insight into how muscles respond to the absence of major scaffold proteins, and whether these transcriptional responses are filament-specific or generic to the tissue. RNA was extracted from the IFMs of 1-2 day old adult male flies of 3 genotypes (Canton-S, Act88FKM88, Mhc7), labeled, and hybridized on Affymetrix GeneChip Drosophila Genome 2.0 microarrays. 9 microarray experiments from 3 independent biological replicates of each genotype. Data was analyzed using RMA method, and significance was tested using SAM version 3.02, with cut-off parameters of 10% FDR and minimum 2-fold change in expression levels.

2015-06-25 | E-GEOD-70252 | biostudies-arrayexpress

Expression data from mouse limb tendon cells during development.

Project description:We have undertaken a screen of mouse limb tendon cells in order to identify molecular pathways involved in tendon development. Mouse limb tendon cells were isolated based on Scleraxis (Scx) expression at different stages of development: E11.5, E12.5 and E14.5 Microarray comparisons were carried out between tendon progenitor and differentiated stages. Forelimbs from E11.5, E12.5 and E14.5 Scx-GFP embryos were collected and dissociated with trypsin to obtain cell suspensions. Scx-positive tendon cells were isolated by FACS. RNA was extracted and Fragmented biotin-labelled cRNA samples were hybridized on Affymetrix Gene Chip Mouse Genome 430 2.0 arrays.

2014-01-18 | E-GEOD-54207 | biostudies-arrayexpress

Expression data from mouse limb tendon cells during development.

2014-01-18 | GSE54207 | GEO

Expression data from rat Achilles tendon-derived stem cells during development

Project description:We have compared the gene expression profile of rat Achilles tendon-derived stem cells in post-natal tendon development. Rat tendon stem/progenitor cells (TSPCs) were isolated at different stages of post-natal development: TSPCs-1d, TSPCs-7d and TSPCs-56d. TSPCs at different post-natal development stages (1d, 7d and 56d) were isolated, cultured and used for microarray analyses at passage 2. All TSPCs in this study were of isolated from more than one individual. Total RNA was extracted and fragmented biotin-tagged cRNA was hybridized to Rat Genome 230 2.0 Array.

2015-07-10 | E-GEOD-68485 | biostudies-arrayexpress

Expression data from rat Achilles tendon-derived stem cells during development

2015-07-10 | GSE68485 | GEO

Tendon injury

Project description:Tendon injury

| PRJNA1027305 | ENA

RNA-seq of achilles tendon from young and old donors to study age-related changes in tendon

Project description:Tendon from young and old donors was used for RNA-Seq analysis. The aim of the study was to identify differentially expressed tendon transcripts in ageing in order to to characterize molecular mechanisms associated with age-related changes in tendon.

2015-04-01 | E-MTAB-2449 | biostudies-arrayexpress

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