Impact of HPV16 E6 and E7 oncogenes together with Chlamydia trachomatis infection on proteome changes in immortalized oral keratinocytes

ABSTRACT: Certain types of Human papilloma viruses (HPV) are the etiological agents for cervical cancer. However, not all infections of high-risk HPVs will finally lead to cancer since most HPV infections are cleared without any consequences. Chlamydia trachomatis is the most prevalent sexual transmitted bacteria and is an obligatory intracellular pathogen exhibiting tropism in endocervical epithelial cells. Over the past decades, C. trachomatis is thought to be a potential co-factor for cervical cancer formation, but there are also studies that did not show such a correlation. To address this question in molecular terms, we stably expressed HPV16 E6 and E7 in spontaneously immortalized NOKs (normal oral keratinocytes) and performed SILAC (stable isotope labeling by amino acids in cell culture) with or without C. trachomatis infection to study the impact of HPV16 oncogene expression and C. trachomatis infection on host proteome changes.

OTHER RELATED OMICS DATASETS IN: GSE124357

INSTRUMENT(S): Q Exactive

ORGANISM(S): Human Papillomavirus Type 16 Homo Sapiens (human) Chlamydia Trachomatis

TISSUE(S): Cell Culture, Keratinocyte

DISEASE(S): Cervix Carcinoma

SUBMITTER: Jana Klimentova  

LAB HEAD: Martina Niebler

PROVIDER: PXD012186 | Pride | 2019-03-18

REPOSITORIES: Pride