Dataset Information

Glomerular filtrate proteins in acute cardiorenal syndrome

ABSTRACT: Acute cardiorenal syndrome (CRS-1) is a morbid complication of acute cardiovascular disease. Mechanistic investigations have focused on intrarenal cellular signaling induced by ischemia/reperfusion. Additional cardiorenal connector signals have been postulated, but investigation in CRS-1 has been limited by a paucity of animal models and technical limitations precluding discovery studies of glomerular filtrate. To address these limitations, we developed a translational model of CRS-1, cardiac arrest and cardiopulmonary resuscitation (CA/CPR) in mice. Quantitative proteomics using isobaric tagging (10-plex TMT reagents, Thermo Fisher) was performed on 24h urine collections from mice with deficient tubular endocytosis and from littermate controls, before and after CA/CPR. Data acquisition used the SPS MS3 method on a Thermo Fusion Tribrid mass spectrometer for accurate reporter ion signals. The findings confirmed CA/CPR-specific cardiac proteins in urine and identified a novel CA/CPR-specific filtrate component: Cardiac LIM protein (CSRP3).

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Urine

DISEASE(S): Cardiovascular System Disease,Myocardial Ischemia

SUBMITTER: Phillip Wilmarth

LAB HEAD: Dr. Michael P. Hutchens

PROVIDER: PXD012193 | Pride | 2019-02-27

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	HUTC-150_Mouseurine_TMT_55pt3ug_14pctACN_25cm_120min_20160131_OT.raw	Raw
	HUTC-150_Mouseurine_TMT_55pt3ug_17pctACN_25cm_120min_20160131_OT.raw	Raw
	HUTC-150_Mouseurine_TMT_55pt3ug_20pctACN_25cm_120min_20160131_OT.raw	Raw
	HUTC-150_Mouseurine_TMT_55pt3ug_21pctACN_25cm_120min_20160131_OT.raw	Raw
	HUTC-150_Mouseurine_TMT_55pt3ug_22pctACN_25cm_120min_20160131_OT.raw	Raw

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Glomerular filtrate proteins in acute cardiorenal syndrome.

Wakasaki Rumie R Matsushita Katsuyuki K Golgotiu Kirsti K Anderson Sharon S Eiwaz Mahaba B MB Orton Daniel J DJ Han Sang Jun SJ Lee H Thomas HT Smith Richard D RD Rodland Karin D KD Piehowski Paul D PD Hutchens Michael P MP

JCI insight 20190221 4

Acute cardiorenal syndrome (CRS-1) is a morbid complication of acute cardiovascular disease. Heart-to-kidney signals transmitted by "cardiorenal connectors" have been postulated, but investigation into CRS-1 has been limited by technical limitations and a paucity of models. To address these limitations, we developed a translational model of CRS-1, cardiac arrest and cardiopulmonary resuscitation (CA/CPR), and now report findings from nanoscale mass spectrometry proteomic exploration of glomerula ...[more]

PMID: 30829647

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