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Identification of PAC1 interactome in activated T cells

ABSTRACT: To examine the molecular mechanisms by which PAC1 suppresses T cell response to antigen, we conducted immunoprecipitation followed by mass spectrometry to characterize PAC1-associated proteins in activated T cells

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell

SUBMITTER: Liang Liu

LAB HEAD: Yuxin Yin

PROVIDER: PXD012201 | Pride | 2020-01-07

REPOSITORIES: Pride

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Publications

The phosphatase PAC1 acts as a T cell suppressor and attenuates host antitumor immunity.

Dan Lu Liu Liang L Sun Yizhe Y Song Jia J Yin Qi Q Zhang Guangze G Qi Fang F Hu Zixi Z Yang Zeliang Z Zhou Zhe Z Hu Ying Y Zhang Lianhai L Ji Jiafu J Zhao Xuyang X Jin Yan Y McNutt Michael A MA Yin Yuxin Y

Nature immunology 20200113 3

Cancer cells subvert immune surveillance through inhibition of T cell effector function. Elucidation of the mechanism of T cell dysfunction is therefore central to cancer immunotherapy. Here, we report that dual specificity phosphatase 2 (DUSP2; also known as phosphatase of activated cells 1, PAC1) acts as an immune checkpoint in T cell antitumor immunity. PAC1 is selectively upregulated in exhausted tumor-infiltrating lymphocytes and is associated with poor prognosis of patients with cancer. PA ...[more]

PMID: 31932812

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