Proteomics

Dataset Information

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Urinary peptidomics in youths with and without type 1 diabetes


ABSTRACT: Although the early mechanisms responsible for diabetic kidney disease remain unclear, it is widely believed that chronic hyperglycemia disrupts the proteolytic milieu in the diabetic kidney and may contribute to early kidney injury. We thus performed mechanistic peptidomics in type 1 diabetes before the onset of microalbuminuria. In the discovery cohort of 30 participants, we identified 6550 peptides from 753 proteins. After removing false hits and potential contaminants, there were 6323 quantified peptides: 5708 peptides can be found in youths with type 1 diabetes, 5011 in healthy controls, and 4396 common to both groups. After applying a cut-off of 100%, there were 162 peptides found in each and every urine sample. From this subset, fifteen peptides were differentially excreted between youths with type 1 diabetes and their non-diabetic peers (P < 0.05, t-test); five remained significant after Benjamini-Hochberg adjustment (Q < 0.05). Excretion rates of six peptides were validated in a second cohort of thirty participants using parallel reaction monitoring (separate PRIDE file). Interestingly, these peptides originated from a small region near the C-terminus of uromodulin, a kidney-specific protein. In silico analyses of cleavage sites implicated several metallo and serine proteases involved in wound healing. Two of the uromodulin peptides activated NFκB in a TLR4-dependent manner in cultured kidney tubular cells and also promoted neutrophil migration in vitro. In summary, the differential excretion of uromodulin peptides may shed light onto early inflammatory processes in the diabetic kidney.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine

SUBMITTER: Julie Anh Dung Van  

LAB HEAD: Ana Konvalinka

PROVIDER: PXD012210 | Pride | 2020-01-20

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
AdDIT_pep10_Fr1_Inj1.raw Raw
AdDIT_pep10_Fr2_Inj1.raw Raw
AdDIT_pep10_Fr3_Inj1.raw Raw
AdDIT_pep11_Fr1_Inj1.raw Raw
AdDIT_pep11_Fr2_Inj1.raw Raw
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Publications

Peptidomic Analysis of Urine from Youths with Early Type 1 Diabetes Reveals Novel Bioactivity of Uromodulin Peptides <i>In Vitro</i>.

Van Julie A D JAD   Clotet-Freixas Sergi S   Zhou Joyce J   Batruch Ihor I   Sun Chunxiang C   Glogauer Michael M   Rampoldi Luca L   Elia Yesmino Y   Mahmud Farid H FH   Sochett Etienne E   Diamandis Eleftherios P EP   Scholey James W JW   Konvalinka Ana A  

Molecular & cellular proteomics : MCP 20191226 3


Chronic hyperglycemia is known to disrupt the proteolytic milieu, initiating compensatory and maladaptive pathways in the diabetic kidney. Such changes in intrarenal proteolysis are captured by the urinary peptidome. To elucidate the early kidney response to chronic hyperglycemia, we conducted a peptidomic investigation into urines from otherwise healthy youths with type 1 diabetes and their non-diabetic peers using unbiased and targeted mass spectrometry-based techniques. This cross-sectional s  ...[more]

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