Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Quantitative proteomic analysis of hippocampal tissues from mice treated with a CK1δ/ε inhibitor

ABSTRACT: Hippocampal tissues from 3xTg-AD mice treated with a CK1δ/ε inhibitor or vehicle, and non-transgenic mice treated with vehicle, were harvested and processed for proteomic analysis using label-free quantification.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Paula Adler

LAB HEAD: Daniel Figeys

PROVIDER: PXD012281 | Pride | 2019-07-30

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20171019_Paula_circadian_AD_group2-1_hippocampus_M001.raw	Raw
	20171019_Paula_circadian_AD_group2-1_hippocampus_M002.raw	Raw
	20171019_Paula_circadian_AD_group2-1_hippocampus_M003.raw	Raw
	20171019_Paula_circadian_AD_group2-1_hippocampus_M004.raw	Raw
	20171019_Paula_circadian_AD_group2-1_hippocampus_M005.raw	Raw

Items per page:

1 - 5 of 24

Publications

Therapeutic Targeting of Casein Kinase 1δ/ε in an Alzheimer's Disease Mouse Model.

Adler Paula P Mayne Janice J Walker Krystal K Ning Zhibin Z Figeys Daniel D

Journal of proteome research 20190806 9

Sleep disturbances and memory impairment are common symptoms of Alzheimer's disease (AD). Given that the circadian clock regulates sleep, hippocampal function, and neurodegeneration, it represents a therapeutic target against AD. Casein kinase 1δ/ε (CK1δ/ε) are clock regulators and overexpressed in AD brains, making them viable targets to improve sleep and cognition. In this study, we evaluated the therapeutic potential of a small molecule CK1δ/ε inhibitor (PF-670462) in a triple transgenic mous ...[more]

PMID: 31334659

Similar Datasets

Hippocampal circadian proteomes of young and middle-aged mice

Project description:Hippocampal tissues from young and middle-aged C57BL/6J mice were harvested at 4-hour intervals over two days and processed for proteomic analysis using label-free quantification.

2020-05-26 | PXD013364 | Pride

Effects of altered expression and activity levels of casein kinase 1δ and ε on tumor growth and survival of colorectal cancer patients

Project description:Colorectal cancer (CRC) is the fourth leading cause of cancer-related death worldwide due to high apoptotic resistance and metastatic potential. Since mutations as well as deregulation of CK1 isoforms contribute to tumor development and progression, CK1 has become an interesting drug target. In this study, we show that CK1 isoforms are differently expressed in colon tumor cell lines and that growth of these cell lines can be inhibited by CK1-specific inhibitors. Furthermore, expression of CK1δ and ε is changed in colorectal tumors and high CK1ε expression levels significantly correlate with prolonged patients' survival. In addition to changes in CK1δ and ε expression, mutations within exon 3 of CK1δ were detected in colorectal tumors. These mutations influence ATP binding, leading to changes in the kinetic parameters. Overexpression of these mutants in HT29 cells alters their ability to grow anchorage independently. Consistent with these results, these CK1δ mutants lead to differences in proliferation rate and tumor size in xenografts due to changes in gene expression, especially in genes involved in regulation of cell proliferation, cell cycle, and apoptosis. In summary, our results provide evidence that changes in the expression levels of CK1 isoforms in colorectal tumors correlate with the survival of patients and that CK1δ mutations affect growth and proliferation of tumor cells and induced tumor growth in xenografts, leading to the assumption that CK1 isoforms provide interesting targets in new colorectal cancer therapy concepts.

2017-05-19 | GSE57840 | GEO

Pulmonary miRNA expression in C57BL/6 Bom Tac mice (dams) intratracheally instilled with Printex 90 carbon black nanoparticles

Project description:miRNAs were measured in pregant mice exposed to vehicle control (nanopure water) or a cumulative dose of 0.268 mg Printex 90 in vehicle by intratracheal instillation and sacrificed at weaning (26-27 days after exposure). Pregnant mice were exposed to vehicle control (nanopure water) or a cumulative dose of 0.268 mg Printex 90 in vehicle by intratracheal instillation. Doses of 0.067 mg were delivered 4 times in pregnant females on gestational days 7, 10, 15 and 18. Mice were sacrificed at weaning (26-27 days post-exposure).

2012-04-01 | E-GEOD-36170 | biostudies-arrayexpress

Pulmonary miRNA expression in C57BL/6 non-pregnant female mice intratracheally instilled with Printex 90 carbon black nanoparticles

Project description:miRNAs were measured in mice exposed to vehicle control (0.9% NaCl MilliQ water containing 10% v/v acellular BAL) or 0.162 mg Printex 90 in vehicle by intratracheal instillation and sacrificed 1, 3 and 28 days after exposure. C57BL/6 mice were exposed to 0.000 or 0.162 mg Printex 90 and sacrificed 1, 3 and 28 days after exposure. Individual total RNA enriched with small RNA from 6 mice per treatment group (control and 0.162mg) was hybridized to 8 x 15K Agilent miRNA slides.

2012-04-01 | E-GEOD-36171 | biostudies-arrayexpress

Metaproteomic responses of in vitro gut microbiomes to resistant starches

Project description:Resistant starches (RS) are dietary compounds processed by the gut microbiota into metabolites, such as butyrate, that are beneficial to the host. The production of butyrate by the microbiome appears to be affected by the plant source and type of RS as well as the individual’s microbiota. In this study, we used in vitro culture and metaproteomic methods to explore the consistency and variations in individual microbiome's functional responses to three types of RS - RS2(Hi Maize 260), RS3(Novelose 330) and RS4(Fibersym RW). Results showed that RS2 and RS3 significantly altered the levels of protein expression in the individual gut microbiomes, while RS4 did not result in significant protein changes. Significantly elevated protein groups were enriched in carbohydrate metabolism and transport functions of families Eubacteriaceae, Lachnospiraceae and Ruminococcaceae. In addition, Bifidobacteriaceae was significantly increased in response to RS3. We also observed taxon-specific enrichments of starch metabolism and pentose phosphate pathways corresponding to this family. Functions related to starch utilization, ABC transporters and pyruvate metabolism pathways were consistently increased in the individual microbiomes in response to RS2 and RS3; in contrast, the downstream butyrate producing pathway response varied. Our study confirm that different types of RS have markedly variable functional effects on the human gut microbiome, and also found considerable inter-individual differences in microbiome pathway responses.

2021-09-08 | PXD017907 | Pride

Modulation of the transcriptional response to recombinant IFN-Î² by Acetaminophen

Project description:We examined the effect of acetaminophen (APAP) on the transcriptional response induced by interferon-Î² (IFN-Î²). Transcript levels from total RNA isolated rom murine livers were determined using G4121B whole genome arrays. Keywords: Pharmakinetics, drug treatment 6-8 week male CD-1 mice were administered vehicle alone, APAP, IFN-Î² or IFN-Î² + APAP (n=5 per treatment per time point 40 animals total) and were euthanized at 1.5 or 4 hours post-treatment. Agilent 22k whole genome arrays were used to measure changes in mRNA transcripts in the liver mRNA. Stratatagene Universal Mouse RNA was used as a reference.

2010-10-18 | E-GEOD-18949 | biostudies-arrayexpress

mRNA expression profiling in adult mouse liver following benzo(a)pyrene (BaP) treatment [Agilent gene expression array]

Project description:Adult male mice were exposed to 150 mg/kg benzo(a)pyrene (BaP) or solvent for 3 days and sampled 4 or 24 hours after the last dose. mRNA expression levels in adult mouse liver were measured using Agilent arrays. We found widespread changes in mRNA in pathways consistent with known response (xenobiotic metabolism, glutathione). We also found that BaP caused changes in the circadian rhythm pathway. We measured miRNA changes in the same samples and found no evidence for any change in transcription of miRNA as a result of the exposure. Keywords: Toxicology, time course Adult male B6C3F1 mice were exposed to a daily dose of corn oil (vehicle control group) or 150 mg/kg BaP (treatment group) for 3 days (n=5 per group). Mice were sacrificed at 4 h or 24 h following the last dose and liver lobes were extracted and flash frozen. RNA was extracted from median liver lobes and hybridized to the Agilent 4 x 44K mouse DNA arrays using a randomized block design.

2010-10-18 | E-GEOD-18789 | biostudies-arrayexpress

Cardiac gene expression (random section containing both ventricles) in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles (set 1)

Project description:C57BL/6 mice were exposed to vehicle or 0.162 mg carbon black nanoparticles by intratracheal installation and were sacrificed 1, 3 and 28 days post-exposure. Gene expression was investigated in two sets of mice treated according to the identical exposure protocol. MicroRNAs were investigated in the second set of mice. Individual total RNA (200 ng) from 6 mice per treatment group (control and 0.162mg) and Universal Mouse Reference total RNA (Stratagene, Mississauga, ON, Canada) was used to synthesize double-stranded cDNA.

2013-05-02 | E-GEOD-37885 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: quinpirole in the telencephalon

Project description:Sexually mature female goldfish were administered 2 i.p. injections: 1) 0.1% DMSO in 0.6% saline, and; 2) 0.6% saline OR quinpirole (2 ug/g) dissolved in 0.6% saline. A common reference design was used: 4 independent groups of telencephali from sexually mature female goldfish treated with 2 ug/g quinpirole hybridized against a common vehicle control. Slides 8, 16, 24, 40.

2013-03-20 | E-GEOD-14609 | biostudies-arrayexpress

AURATUS goldfish environmental genomics projects: SKF 38393 in the hypothalamus

Project description:Sexually mature female goldfish were administered 2 i.p. injections: 1) 0.1% DMSO in 0.6% saline or SKF 38393 (40 µg/g) dissolved in 0.1% DMSO in 0.6% saline, and; 2) 0.6% saline. A common reference design was used: 4 independent groups of hypothalami from sexually mature female goldfish treated with 40 ug/g SKF 38393 hybridized against a common vehicle control. Slides 9, 19, 33, 44.

2011-08-21 | E-GEOD-14607 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data