Dataset Information

Proteomic analysis of Dpy19l2-deficient human globozoospermia reveals multiple molecular defects

ABSTRACT: Globozoospermia is a severe teratozoospermia characterized by round-headed spermatozoa without acrosomes, resulting in male infertility. The clinical treatment of globozoospermia involves primarily intracytoplasmic sperm injection because of the low fertilization rate. The deletion of Dpy19l2 is known to be the major cause of globozoospermia. The defects in Dpy19l2-deficient human sperm at the protein level were characterized by performing a isotope-based quantitative proteomic analysis between Dpy19l2-deficient globozoospermic spermatozoa and normal controls. A total of 2,549 proteins were identified, including 495 differentially expressed proteins (fold-change >2), of which 322 were upregulated and 123 were downregulated. The levels of several important proteins, including SPACA 1, IZUMO1, ZPBP1, and PLCZ1, were decreased in globozoospermic sperm. Bioinformatics analysis indicated the Dpy19l2-deficient sperm presented molecular defects in acrosome, chromatin, sperm-egg interaction, and fertilization. This study may provide insights into the mechanism of globozoospermia and help develop better treatments to increase the success rate of fertilization.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Testis

SUBMITTER: Xiaofei Liu

LAB HEAD: Xuejiang Guo

PROVIDER: PXD012302 | Pride | 2019-08-15

REPOSITORIES: Pride

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			Action	DRS
	human-roundsperm-TMT6plex-SCX-gys-205min-1-2.raw	Raw
	human-roundsperm-TMT6plex-SCX-gys-205min-11-12.raw	Raw
	human-roundsperm-TMT6plex-SCX-gys-205min-13-14.raw	Raw
	human-roundsperm-TMT6plex-SCX-gys-205min-15-16.raw	Raw
	human-roundsperm-TMT6plex-SCX-gys-205min-17-18.raw	Raw

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Proteomic Analysis of Dpy19l2-Deficient Human Globozoospermia Reveals Multiple Molecular Defects.

Guo Yueshuai Y Jiang Jiayin J Zhang Haotian H Wen Yang Y Zhang Hao H Cui Yiqiang Y Tian Jianyu J Jiang Min M Liu Xiaofei X Wang Gaigai G Li Yan Y Hu Zhibin Z Zhou Zuomin Z Sha Jiahao J Chen Daozhen D Yang Xiaoyu X Guo Xuejiang X

Proteomics. Clinical applications 20190904 6

<h4>Purpose</h4>To investigate the differences in protein expression between Dpy19l2-deficient human globozoospermia and normozoospermia.<h4>Experimental design</h4>Human sperm samples from three globozoospermic donors with Dpy19l2 deletion and three normal controls are subjected to TMT quantitative technology. SPESP1, HIST1H4A, and LYZL1 are randomly selected for western blotting analysis. GO annotations are performed using the Database for Annotation, Visualization, and Integrated Discovery.<h ...[more]

PMID: 31424156

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