Dataset Information

Phosphoproteomics of endothelin signalling

ABSTRACT: Time resolved phosphorylation changes were measured the melanoma cell lines A2058 and UACC257. An endothelin B receptor knockout of UACC257 was processed in parallel. Protein abundance was also compared at the latest time point of the study (90 min) using DIA-SWATH

INSTRUMENT(S): TripleTOF 6600, LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Melanocyte

SUBMITTER: Alexander Schäfer

LAB HEAD: Ruedi Aebersold

PROVIDER: PXD012316 | Pride | 2019-10-09

REPOSITORIES: pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	A2058_iprophet.pep.xml	Pepxml
	OpenSWATH_output.tsv	Tabular
	Phospho_DDA_A2058_0min_Ctr_1.mzXML.mzXML	Mzxml
	Phospho_DDA_A2058_0min_Ctr_2.mzXML.mzXML	Mzxml
	Phospho_DDA_A2058_0min_Ctr_3.mzXML.mzXML	Mzxml

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Publications

Elucidating essential kinases of endothelin signalling by logic modelling of phosphoproteomics data.

Schäfer Alexander A Gjerga Enio E Welford Richard Wd RW Renz Imke I Lehembre Francois F Groenen Peter Ma PM Saez-Rodriguez Julio J Aebersold Ruedi R Gstaiger Matthias M

Molecular systems biology 20190801 8

Endothelins (EDN) are peptide hormones that activate a GPCR signalling system and contribute to several diseases, including hypertension and cancer. Current knowledge about EDN signalling is fragmentary, and no systems level understanding is available. We investigated phosphoproteomic changes caused by endothelin B receptor (ENDRB) activation in the melanoma cell lines UACC257 and A2058 and built an integrated model of EDNRB signalling from the phosphoproteomics data. More than 5,000 unique phos ...[more]

PMID: 31464372

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