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Coacervation of Lipid Bilayer in Natural Cell Membranes for Extraction and Enrichment of Proteins in Proteomics Studies

ABSTRACT: This is the first report on fluoroalcohol-induced coacervation of lipid components in natural cell membranes and incorporation of this novel process for extraction, fractionation, and enrichment of proteins in proteomics workflow.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Amir Koolivand

LAB HEAD: Morteza Khaledi

PROVIDER: PXD012349 | Pride | 2019-03-28

REPOSITORIES: pride

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Coacervation of Lipid Bilayer in Natural Cell Membranes for Extraction, Fractionation, and Enrichment of Proteins in Proteomics Studies.

Koolivand Amir A Azizi Mohammadmehdi M O'Brien Ariel A Khaledi Morteza G MG

Journal of proteome research 20190313 4

This is the first report where hexafluoroisopropanol (HFIP) was used to induce the coacervation of lipid components in natural cell membranes that would concomitantly result in solubilization, extraction, and enrichment of hydrophobic proteins (e.g., integral membrane proteins, IMP) into the coacervate phase, and extraction of hydrophilic proteins in a separate aqueous phase. The incorporation of this innovative approach in the proteomics workflow would allow the fractionation of proteins in sep ...[more]

PMID: 30810315

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