Proteomics

Dataset Information

0

Alternative Splicing of Fibronectin Regulates Composition of the Inflamed Arterial Wall

ABSTRACT: Exposure of the arterial endothelium to low and disturbed flow is a risk factor for the erosion and rupture of atherosclerotic plaques and aneurysms. Circulating and locally produced proteins are known to contribute to an altered matrix composition at the site of lesions, and to contribute to inflammatory processes within the lesions by altering the sub-endothelial matrix. We have previously shown that immune-cell regulated alternative splicing of Fibronectin (FN) protects against flow-induced hemorrhage. Here, we perform quantitative proteomic analysis of enriched extracellular matrix preparations from murine carotid arteries exposed to low and disturbed flow in vivo and examine serum derived and endothelial cell contributions to the sub-endothelial matrix in vitro. Our results reveal the extent of the dynamic alterations in extracellular matrix composition in the acute response to low and disturbed flow, and show how changes in the splicing of FN, a common response in vascular inflammation and remodeling, affects matrix composition.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

SUBMITTER: Richard Schiavoni  

LAB HEAD: Richard P Schiavoni

PROVIDER: PXD012474 | Pride | 2022-02-15

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
150607_139_NJ_a_fr1.mgf Mgf
150607_139_NJ_a_fr1.mzid.gz Mzid
150607_139_NJ_a_fr1.pride.mgf.gz Mgf
150607_139_NJ_a_fr1.pride.mztab.gz Mztab
150607_139_NJ_a_fr1.raw Raw
Items per page:
1 - 5 of 504
altmetric image

Publications

Alternative Splicing of FN (Fibronectin) Regulates the Composition of the Arterial Wall Under Low Flow.

Murphy Patrick A PA   Jailkhani Noor N   Nicholas Sarah-Anne SA   Del Rosario Amanda M AM   Balsbaugh Jeremy L JL   Begum Shahinoor S   Kimble Amy A   Hynes Richard O RO  

Arteriosclerosis, thrombosis, and vascular biology 20201119 1

<h4>Objective</h4>Exposure of the arterial endothelium to low and disturbed flow is a risk factor for the erosion and rupture of atherosclerotic plaques and aneurysms. Circulating and locally produced proteins are known to contribute to an altered composition of the extracellular matrix at the site of lesions, and to contribute to inflammatory processes within the lesions. We have previously shown that alternative splicing of FN (fibronectin) protects against flow-induced hemorrhage. However, th  ...[more]

Similar Datasets

Transcriptomics Genome-wide analysis of mechanosensitive genes using in vivo model of mouse carotid artery endothelium exposed to disturbed flow
2011-03-10 | GSE20741 | GEO
Transcriptomics Genome-wide analysis of mechanosensitive genes using in vivo model of mouse carotid artery endothelium exposed to disturbed flow
2011-03-10 | E-GEOD-20741 | biostudies-arrayexpress
Proteomics Flow-induced reorganization of laminin-integrin networks within the endothelial basement membrane uncovered by proteomics
2020-04-29 | PXD014526 | Pride
Transcriptomics Identification of novel mechanosensitive genes differentially regulated at 48 h timepoint in vivo using mouse carotid artery endothelium exposed to disturbed blood flow
2014-01-13 | GSE52243 | GEO
Transcriptomics Flow-Mediated Modulation of the Endothelial Cell Lipidome
2024-07-01 | GSE266437 | GEO
Transcriptomics Identification of novel mechanosensitive genes differentially regulated at 48 h timepoint in vivo using mouse carotid artery endothelium exposed to disturbed blood flow
2014-01-13 | E-GEOD-52243 | biostudies-arrayexpress
Transcriptomics Alternative RNA Splicing in the Endothelium Mediated in Part by Rbfox2 Regulates the Arterial Response to Low Flow
2018-01-08 | GSE101826 | GEO
Transcriptomics Deep-sequencing of endothelial-enriched RNAs from carotid arteries of C57 Bl6 mice subjected to partial carotid ligation (PCL) to determine the effect of disturbed blood flow and aging
2024-02-07 | GSE197366 | GEO
Transcriptomics 3D vascularized proximal tubule model improves cell phenotype
2022-03-20 | GSE198822 | GEO
Transcriptomics Transcription profiling of pig aortic endothelium taken from regions of disturbed and undisturbed flow
2006-07-21 | E-CBIL-17 | biostudies-arrayexpress