Cysteine nitrosylation analysis in SH-SY5Y cells
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: 
Ruzanna Mnatsakanyan
LAB HEAD: René P. Zahedi
PROVIDER: PXD012485 | Pride | 2019-05-21
REPOSITORIES: Pride
Dataset's files
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Publications
Proteome-wide detection of S-nitrosylation targets and motifs using bioorthogonal cleavable-linker-based enrichment and switch technique.
Mnatsakanyan Ruzanna R Markoutsa Stavroula S Walbrunn Kim K Roos Andreas A Verhelst Steven H L SHL Zahedi René P RP
Nature communications 20190516 1
Cysteine modifications emerge as important players in cellular signaling and homeostasis. Here, we present a chemical proteomics strategy for quantitative analysis of reversibly modified Cysteines using bioorthogonal cleavable-linker and switch technique (Cys-BOOST). Compared to iodoTMT for total Cysteine analysis, Cys-BOOST shows a threefold higher sensitivity and considerably higher specificity and precision. Analyzing S-nitrosylation (SNO) in S-nitrosoglutathione (GSNO)-treated and non-treate ...[more]
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