Proteomics

Dataset Information

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Investigation of Oligodendrocyte Precursor Cell Differentiation by Quantitative Proteomics


ABSTRACT: Oligodendrocytes, the myelinating cells of the central nervous system, are essential for correct brain function. They originate from oligodendrocyte precursor cells (OPCs) through a differentiation process which is only incompletely understood and impaired in a variety of demyelinating diseases. Better knowledge of this differentiation holds the promise to develop novel therapies for these disorders. We investigated the differentiation of rat oligodendrocyte precursor cells to oligodendrocytes in vitro. After confirmation of differentiation by immunohistochemical analysis using cell type-specific marker proteins, we conducted a quantitative proteomics study using tandem mass tags (TMT). We investigated four time points of differentiation covering early, intermediate, and late stages. Data analysis was perfomed using Mascot and Maxquant.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain, Oligodendrocyte Precursor Cell

SUBMITTER: Carmen Schoor  

LAB HEAD: Dr. Dominic Winter

PROVIDER: PXD012544 | Pride | 2019-06-21

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
OPC_Maxquant_evidence.txt Txt
OPC_Maxquant_peptides.txt Txt
OPC_Maxquant_proteinGroups.txt Txt
OPCrat_5Replicates_TopN12.msf Msf
Replicate1Fraction1.raw Raw
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Publications

Investigation of Oligodendrocyte Precursor Cell Differentiation by Quantitative Proteomics.

Schoor Carmen C   Brocke-Ahmadinejad Nahal N   Gieselmann Volkmar V   Winter Dominic D  

Proteomics 20190703 14


Oligodendrocytes, the myelinating cells of the central nervous system, are essential for correct brain function. They originate from oligodendrocyte precursor cells through a differentiation process which is only incompletely understood and impaired in a variety of demyelinating diseases. Better knowledge of this differentiation holds the promise to develop novel therapies for these disorders. The differentiation of rat oligodendrocyte precursor cells to oligodendrocytes in vitro is investigated  ...[more]

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