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A Simple Cross-Linking/Mass Spectrometry Workflow to Study System-Wide Protein Interactions in Drosophila Embryos

ABSTRACT: We provide a cross-linking/MS workflow that can be applied to complex systems. The software tool MeroX 2.0 can be used to identify cross-linked peptide on a proteome-wide level. We applied the workflow to extracts of Drosophila embryos and identified 5,129 unique cross-linked residue pairs in biological triplicates.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Embryo

SUBMITTER: Michael Götze

LAB HEAD: Andrea Sinz

PROVIDER: PXD012546 | Pride | 2019-07-09

REPOSITORIES: Pride

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			Action	DRS
	DP.fasta	Fasta
	DP.zdb	Other
	EC.fasta	Fasta
	EC.zdb	Other
	ID.fasta	Fasta

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Publications

A Simple Cross-Linking/Mass Spectrometry Workflow for Studying System-wide Protein Interactions.

Götze Michael M Iacobucci Claudio C Ihling Christian H CH Sinz Andrea A

Analytical chemistry 20190719 15

We present a cross-linking/mass spectrometry workflow for performing proteome-wide cross-linking analyses within 1 week. The workflow is based on the commercially available mass spectrometry-cleavable cross-linker disuccinimidyl dibutyric urea and can be employed by every lab having access to a mass spectrometer with tandem mass spectrometry capabilities. We provide an updated version 2.0 of the freeware software tool MeroX, available at www.StavroX.com , that allows us to conduct fully automate ...[more]

PMID: 31283178

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