Dataset Information

Progressive Changes in Human Follicular Fluid Composition over the Course of Ovulation: Quantitative Proteomic Analyses.

ABSTRACT: The composition of follicular fluid reflects crucial paracrine signalling between granulosa- and theca cells and the oocyte. Dynamic changes in the protein composition of human follicular fluid across multiple time points in the period where final meiotic maturation and release of the oocyte take place are previously undescribed. Twenty-five women undergoing IVF- or ICSI treatment in a standard antagonist protocol with agonist ovulation trigger were included in this prospective cohort study. From each patient one follicle was aspirated by transvaginal ultrasound guided puncture at one of five specific time points either before ovulation induction (T=0) or 12-, 17-, 32- or 36 hours after ovulation induction (five patients/time point). Proteomics was carried out by liquid chromatography-mass spectrometry. In total, 400 proteins were identified (FDR<0.05) and 30 were significantly regulated across time points (one-way ANOVA, adjusted p<0.05). Compared to compiled human plasma proteome sets, 47 proteins were unique to follicular fluid. Enriched biological functions among differentially expressed proteins included immune functions, wound healing and functions related to blood coagulation (FDR<0.02). The most profound changes occurred shortly after ovulation induction. We confirmed parallel protein expression to known granulosa cell mRNA changes and described many hitherto unknown protein expression profiles during ovulation. Thus, the study endorsed important biological functions of some proteins and suggested additional proteins, which may be crucial to intrafollicular signalling and oocyte competence that should be further investigated.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Follicular Fluid

SUBMITTER: Indira Pla Parada

LAB HEAD: Johan Malm

PROVIDER: PXD012561 | Pride | 2019-07-29

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20180718_BFF01_36h-033_DDA.raw	Raw
	20180718_BFF02_12h-017_DDA.raw	Raw
	20180718_BFF03__0h-041_DDA.raw	Raw
	20180718_BFF04_17h-027_DDA.raw	Raw
	20180718_BFF05_32h-024_DDA.raw	Raw

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Progressive changes in human follicular fluid composition over the course of ovulation: quantitative proteomic analyses.

Poulsen Liv la Cour LC Pla Indira I Sanchez Aniel A Grøndahl Marie Louise ML Marko-Varga György G Yding Andersen Claus C Englund Anne Lis Mikkelsen ALM Malm Johan J

Molecular and cellular endocrinology 20190726

Follicular fluid (FF) acts as a vehicle for paracrine signalling between somatic cells of the follicle and the oocyte. To investigate changes in the protein composition of FF during ovulation, we conducted a prospective cohort study including 25 women undergoing fertility treatment. Follicular fluid was aspirated either before or 12, 17, 32 or 36 h after induction of ovulation (five patients per time point). Liquid chromatography-mass spectrometry was used to identify and quantify FF proteins. I ...[more]

PMID: 31356852

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