Proteomics,Multiomics

Dataset Information

0

RNA degradosome components purified from Mycobacterium tuberculosis


ABSTRACT: The dataset accompanying a publication describing the RNA degradosome components in Mycobacterium tuberculosis (Mtb). Individual RNA degradosome components from Mtb (PNPase, RNase E, RNase J, RhlE and Enolase) were tagged with eGFP and used as baits to pull-down their interaction partners. The protein-complexes were purified by affinity chromatography, using previously published protocols, and submitted to LC-MS analysis.

OTHER RELATED OMICS DATASETS IN: GSE126286

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mycobacterium Tuberculosis H37rv

SUBMITTER: Przemyslaw Plocinski  

LAB HEAD: Andrzej Dziembowski

PROVIDER: PXD012662 | Pride | 2019-04-01

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
40114341lau_PNP.raw Raw
40114342lau_enolase.raw Raw
40114343lau_RhlE.raw Raw
40214344lau_RnJ.raw Raw
40214345lau_RNE.raw Raw
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Publications

Proteomic and transcriptomic experiments reveal an essential role of RNA degradosome complexes in shaping the transcriptome of Mycobacterium tuberculosis.

Płociński Przemysław P   Macios Maria M   Houghton Joanna J   Niemiec Emilia E   Płocińska Renata R   Brzostek Anna A   Słomka Marcin M   Dziadek Jarosław J   Young Douglas D   Dziembowski Andrzej A  

Nucleic acids research 20190601 11


The phenotypic adjustments of Mycobacterium tuberculosis are commonly inferred from the analysis of transcript abundance. While mechanisms of transcriptional regulation have been extensively analysed in mycobacteria, little is known about mechanisms that shape the transcriptome by regulating RNA decay rates. The aim of the present study is to identify the core components of the RNA degradosome of M. tuberculosis and to analyse their function in RNA metabolism. Using an approach involving cross-l  ...[more]

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