Proteomics

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Integrative proteomic and phosphoproteomics analysis of human ischemic and dilated cardiomyopathic explant tissues identifies etiology-specific phosphorylation patterns and signaling pathways

ABSTRACT: Myocardial tissue samples were explanted from the explanted left ventricle of heart failure patients with DCM (n=4), left ventricular infarct, peri-, and non-infarct regions of HF patients with ICM (n=4) at the time of their heart transplant surgeries, and the left ventricle of age- and sex-matched non-failing controls (NFC, n=4). Comparative quantitative analysis was performed on three independent sets (DCM vs. NFC, infarct vs. non-infarct, and peri- vs. non-infarct) labeled with 10-plex tandem mass tags. Following enrichment of phosphorylated peptides, the flow-through and eluted fractions were collected separately and subjected to LC-MS/MS (liquid chromatography-tandem mass spectrometry) on a Q-Exactive HF for global proteomics and phosphoproteomics profiling respectively.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Heart, Cardiac Muscle Cell

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Uros Kuzmanov  

LAB HEAD: Anthony Orlando Gramolini

PROVIDER: PXD012664 | Pride | 2023-11-20

REPOSITORIES: Pride

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Dataset's files

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Action DRS
DCMvsNFC_PHOSPHO_HILIC2_1.raw Raw
DCMvsNFC_PHOSPHO_HILIC2_10.raw Raw
DCMvsNFC_PHOSPHO_HILIC2_2.raw Raw
DCMvsNFC_PHOSPHO_HILIC2_3.raw Raw
DCMvsNFC_PHOSPHO_HILIC2_4.raw Raw
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Publications

Proteomics and phosphoproteomics of failing human left ventricle identifies dilated cardiomyopathy-associated phosphorylation of CTNNA3.

Reitz Cristine J CJ   Tavassoli Marjan M   Kim Da Hye DH   Shah Saumya S   Lakin Robert R   Teng Allen C T ACT   Zhou Yu-Qing YQ   Li Wenping W   Hadipour-Lakmehsari Sina S   Backx Peter H PH   Emili Andrew A   Oudit Gavin Y GY   Kuzmanov Uros U   Gramolini Anthony O AO  

Proceedings of the National Academy of Sciences of the United States of America 20230501 19

The prognosis and treatment outcomes of heart failure (HF) patients rely heavily on disease etiology, yet the majority of underlying signaling mechanisms are complex and not fully elucidated. Phosphorylation is a major point of protein regulation with rapid and profound effects on the function and activity of protein networks. Currently, there is a lack of comprehensive proteomic and phosphoproteomic studies examining cardiac tissue from HF patients with either dilated dilated cardiomyopathy (DC  ...[more]

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