Proteomics

Dataset Information

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Oxidative Stress Biomarker of Type 2 Diabetes Mellitus

ABSTRACT: In this project, using discovery-based mass spectrometry approach and adopting data-dependent LC-MS/MS, we detected and identified modified peptides in complex clinical plasma samples and developed first-level biomarker verification strategy. Further, using liquid chromatography-multiple reaction monitoring mass spectrometry (LC-MRM-MS) based targeted approach which virtually quantifies irreversible oxidation at any targeted cysteine, we quantified cysteine tri-oxidation in HSA in diabetes patients. Site-specific tri-oxidized HSA could be a potential biomarker of diabetes and provide directive of disease mechanism.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Blood Plasma

DISEASE(S): Type 2 Diabetes Mellitus

SUBMITTER: Sunil Adav  

LAB HEAD: Prof Sze S K

PROVIDER: PXD012667 | Pride | 2020-05-26

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
170508_Alb_Modified_Std_717_A1.raw Raw
170508_Alb_Modified_Std_717_B1.raw Raw
170508_Alb_Modified_Std_951_A1.raw Raw
170508_Alb_Modified_Std_951_B1.raw Raw
170601_Alb_DB_TTSH_Disease1_A01.raw Raw
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Publications

Serum albumin cysteine trioxidation is a potential oxidative stress biomarker of type 2 diabetes mellitus.

Paramasivan Selvam S   Adav Sunil S SS   Ngan SoFong Cam SC   Dalan Rinkoo R   Leow Melvin Khee-Shing MK   Ho Hee Hwa HH   Sze Siu Kwan SK  

Scientific reports 20200415 1

Metabolic disorders in T2DM generate multiple sources of free radicals and oxidative stress that accelerate nonenzymatic degenerative protein modifications (DPMs) such as protein oxidation, disrupt redox signaling and physiological function, and remain a major risk factor for clinical diabetic vascular complications. In order to identify potential oxidative biomarkers in the blood plasma of patients with T2DM, we used LC-MS/MS-based proteomics to profile plasma samples from patients with T2DM an  ...[more]

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