Proteomics

Dataset Information

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The histone chaperone CAF-1 cooperates with the DNA methyltransferases to maintain Cd4 silencing in cytotoxic T cells


ABSTRACT: FLAG IP-MS of transfected HEK293 cells with FLAG tagged mouse Chaf1a constructs (T1, T10) or empty vector (EV).

OTHER RELATED OMICS DATASETS IN: GSE126470GSE128245GSE126613GSE126471GSE126472

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Charles Ng  

LAB HEAD: Dan Littman

PROVIDER: PXD012669 | Pride | 2019-04-18

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
031617_CombinedProteins_Littman.xlsx Xlsx
032417_E_ja_EV.raw Raw
032417_E_ja_T1.raw Raw
032417_E_ja_T10.raw Raw
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Publications

The histone chaperone CAF-1 cooperates with the DNA methyltransferases to maintain <i>Cd4</i> silencing in cytotoxic T cells.

Ng Charles C   Aichinger Martin M   Nguyen Tung T   Au Christy C   Najar Tariq T   Wu Lin L   Mesa Kai R KR   Liao Will W   Quivy Jean-Pierre JP   Hubert Benjamin B   Almouzni Genevieve G   Zuber Johannes J   Littman Dan R DR  

Genes & development 20190411 11-12


The transcriptional repression of alternative lineage genes is critical for cell fate commitment. Mechanisms by which locus-specific gene silencing is initiated and heritably maintained during cell division are not clearly understood. To study the maintenance of silent gene states, we investigated how the <i>Cd4</i> gene is stably repressed in CD8<sup>+</sup> T cells. Through CRISPR and shRNA screening, we identified the histone chaperone CAF-1 as a critical component for <i>Cd4</i> repression.  ...[more]

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