Dataset Information

Semi-quantitative proteomics enables mapping of global neutrophil dynamics following influenza virus infection

ABSTRACT: Lung neutrophils are causally associated with IAV-induced disease severity. Less is known about the repertoire of lethal IAV-associated neutrophil proteins or about how global changes in different neutrophil compartments are coordinated following lethal IAV infection. Here, we use semi-quantitative proteomics to characterize dynamic alterations in BM, blood and lung neutrophils at homeostasis or following a lethal IAV infection, with a secondary aim of identifying lung neutrophil-derived proteins which are selectively induced following IAV infection. Our findings identify bone marrow neutrophil maturation as the key site of anti-viral activity induction, with further upregulation or release of both anti-viral and antimicrobial effectors occurring following lung tissue infiltration.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Bronchoalveolar Lavage, Neutrophil, Bone Marrow, Blood

DISEASE(S): Influenza

SUBMITTER: Chuanxin Liu

LAB HEAD: Weisan Chen

PROVIDER: PXD012681 | Pride | 2019-05-29

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Experiment_1_OxidationMSites.txt	Txt
	Experiment_1_REP01_F1.raw	Raw
	Experiment_1_REP01_F10.raw	Raw
	Experiment_1_REP01_F11.raw	Raw
	Experiment_1_REP01_F12.raw	Raw

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Publications

Semiquantitative Proteomics Enables Mapping of Murine Neutrophil Dynamics following Lethal Influenza Virus Infection.

Liu Chuanxin C Oveissi Sara S Downs Rachael R Kirby Jason J Nedeva Christina C Puthalakath Hamsa H Faou Pierre P Duan Mubing M Chen Weisan W

Journal of immunology (Baltimore, Md. : 1950) 20190715 4

Neutrophils are rapidly deployed innate immune cells, and excessive recruitment is causally associated with influenza-induced pathologic conditions. Despite this, the complete set of influenza lethality-associated neutrophil effector proteins is currently unknown. Whether the expression of these proteins is predetermined during bone marrow (BM) neutrophil maturation or further modulated by tissue compartment transitions has also not been comprehensively characterized at a proteome-wide scale. In ...[more]

PMID: 31308090

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