Dataset Information

Proteomics of muscle and bone marrow samples from the 28,000-year-old remains of a woolly mammoth (Mammuthus primigenius).

ABSTRACT: The 28,000-year-old remains of a woolly mammoth, named ‘Yuka’, were found in Siberian permafrost. We performed proteomic analyses of muscle and bone marrow samples obtained from the remains to gain information about the repertoire and modifications of proteins.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mammuthus Primigenius Elephas Maximus

TISSUE(S): Muscle, Bone Marrow

SUBMITTER: Kouhei Nagai

LAB HEAD: Akira Iritani

PROVIDER: PXD012741 | Pride | 2019-03-13

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	150316HM5ul-150min100sec.wiff	Wiff
	150316HM5ul-150min100sec.wiff.scan	Wiff
	150316HM5ul-150min50sec.wiff	Wiff
	150316HM5ul-150min50sec.wiff.scan	Wiff
	2-M-Bone_20x100msec_6ug_145min_001.wiff	Wiff

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Publications

Signs of biological activities of 28,000-year-old mammoth nuclei in mouse oocytes visualized by live-cell imaging.

Yamagata Kazuo K Nagai Kouhei K Miyamoto Hiroshi H Anzai Masayuki M Kato Hiromi H Miyamoto Kei K Kurosaka Satoshi S Azuma Rika R Kolodeznikov Igor I II Protopopov Albert V AV Plotnikov Valerii V VV Kobayashi Hisato H Kawahara-Miki Ryouka R Kono Tomohiro T Uchida Masao M Shibata Yasuyuki Y Handa Tetsuya T Kimura Hiroshi H Hosoi Yoshihiko Y Mitani Tasuku T Matsumoto Kazuya K Iritani Akira A

Scientific reports 20190311 1

The 28,000-year-old remains of a woolly mammoth, named 'Yuka', were found in Siberian permafrost. Here we recovered the less-damaged nucleus-like structures from the remains and visualised their dynamics in living mouse oocytes after nuclear transfer. Proteomic analyses demonstrated the presence of nuclear components in the remains. Nucleus-like structures found in the tissue homogenate were histone- and lamin-positive by immunostaining. In the reconstructed oocytes, the mammoth nuclei showed th ...[more]

PMID: 30858410

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