Proteomics

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Plant Cell Wall Proteomics: A Focus on Monocot Species, Brachypodium distachyon, Saccharum spp. and Oryza sativa


ABSTRACT: We performed cell wall proteins (CWPs) extraction and analysis from sugarcane 7-month-old young and mature leaves and basal and apical internodes in order to point the differences regarding the cell wall proteome composition in these specific organs and diverse developmental stages. We used two different extraction methods for leaves CWPs: destructive and non-destructive. For internodes, only the non-destructive technique was used. The collection of CWPs was identified through LC-MS/MS analyses. Altogether, 110 sugarcane CWPs were identified and classified into eight functional categories. Sugarcane has one of the highest coverage of CWPs identified by mass spectrometry, reaching 283.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Saccharum Hybrid Cultivar Sp80-3280

TISSUE(S): Plant Cell, Leaf, Shoot

SUBMITTER: Thais Cataldi  

LAB HEAD: Carlos Alberto Labate, Maria Juliana Calderan-Rodrigues, Thaís Regiani Cataldi

PROVIDER: PXD012761 | Pride | 2019-04-24

REPOSITORIES: Pride

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Publications

Plant Cell Wall Proteomics: A Focus on Monocot Species, <i>Brachypodium distachyon</i>, <i>Saccharum</i> spp. and <i>Oryza sativa</i>.

Calderan-Rodrigues Maria Juliana MJ   Guimarães Fonseca Juliana J   de Moraes Fabrício Edgar FE   Vaz Setem Laís L   Carmanhanis Begossi Amanda A   Labate Carlos Alberto CA  

International journal of molecular sciences 20190423 8


Plant cell walls mostly comprise polysaccharides and proteins. The composition of monocots' primary cell walls differs from that of dicots walls with respect to the type of hemicelluloses, the reduction of pectin abundance and the presence of aromatic molecules. Cell wall proteins (CWPs) differ among plant species, and their distribution within functional classes varies according to cell types, organs, developmental stages and/or environmental conditions. In this review, we go deeper into the fi  ...[more]

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