Dataset Information

Proteomic analysis of dafachronic acid-associated alterations in Haemonchus contortus

ABSTRACT: To investigate the molecular changes (protein level) associated with dafachronic acid and the nuclear hormone receptor DAF-12 in Haemonchus contortus following exsheathment of the thrid-stage larvae, protein was extracted from replicates (n = 4), processed and analysed using LC-MS/MS.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Haemonchus Contortus (barber Pole Worm)

TISSUE(S): Whole Body, Somatic Cell

DISEASE(S): Parasitic Helminthiasis Infectious Disease

SUBMITTER: Tao Wang

LAB HEAD: Robin Beat Gasser

PROVIDER: PXD012878 | Pride | 2019-11-13

REPOSITORIES: Pride

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Publications

Dafachronic acid promotes larval development in Haemonchus contortus by modulating dauer signalling and lipid metabolism.

Ma Guangxu G Wang Tao T Korhonen Pasi K PK Young Neil D ND Nie Shuai S Ang Ching-Seng CS Williamson Nicholas A NA Reid Gavin E GE Gasser Robin B RB

PLoS pathogens 20190723 7

Here, we discovered an endogenous dafachronic acid (DA) in the socioeconomically important parasitic nematode Haemonchus contortus. We demonstrate that DA promotes larval exsheathment and development in this nematode via a relatively conserved nuclear hormone receptor (DAF-12). This stimulatory effect is dose- and time-dependent, and relates to a modulation of dauer-like signalling, and glycerolipid and glycerophospholipid metabolism, likely via a negative feedback loop. Specific chemical inhibi ...[more]

PMID: 31335899

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			Action	DRS
	0_1control0h.raw	Raw
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	1_1da24h.raw	Raw