Dataset Information

The dual prey-inactivation strategy of spiders - In-depth venomic analysis of Cupiennius salei

ABSTRACT: Most knowledge on spider venoms concerns neurotoxins acting on ion channels, whereas proteins and their significance for the envenomation process are neglected. The comprehensive analysis presented here of the venom gland transcriptome and proteome of Cupiennius salei with a focus on proteins and cysteine-containing peptides offers new insight into the structure and function of spider venom, presented here as dual prey-inactivation strategy. After venom injection, many enzymes and proteins, dominated by α-amylase, angiotensin-converting enzyme, and cysteine-rich secretory proteins, interact with main metabolic pathways, leading to major disturbance of the cellular homeostasis. Hyaluronidase and cytolytic peptides destroy tissue and membranes, thus supporting the spread of other venom compounds. We detected 81 transcripts of neurotoxins from 13 peptide families, whereof two families comprise 93.7% of all cysteine-containing peptides. This raises the question of the importance of the other low-expressed peptide families. The identification of a venom gland-specific defensin-like peptide and an aga-toxin-like peptide in the hemocytes offers an important clue on the recruitment and neofunctionalization of body proteins and peptides as the origin of toxins.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Cupiennius Salei (american Wandering Spider)

TISSUE(S): Venom

SUBMITTER: Nicolas Langenegger

LAB HEAD: Lucia Kuhn-Nentwig

PROVIDER: PXD012886 | Pride | 2019-03-25

REPOSITORIES: Pride

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			Action	DRS
	20180328_f2_topdown_OTMS2_EThcD_NL_i02.mzXML	Mzxml
	20180328_f2_topdown_OTMS2_EThcD_NL_i02.raw	Raw
	20180328_f2_topdown_OTMS2_EThcD_NL_i02_ms2.FORM_OUTPUT_TABLE	Other
	20180328_f2_topdown_OTMS2_EThcD_NL_i02_ms2.OUTPUT_TABLE	Other
	20180411_f3_topdown_OTMS2_EThcD_NL_i02.mzXML	Mzxml

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The Dual Prey-Inactivation Strategy of Spiders-In-Depth Venomic Analysis of <i>Cupiennius salei</i>.

Kuhn-Nentwig Lucia L Langenegger Nicolas N Heller Manfred M Koua Dominique D Nentwig Wolfgang W

Toxins 20190319 3

Most knowledge of spider venom concerns neurotoxins acting on ion channels, whereas proteins and their significance for the envenomation process are neglected. The here presented comprehensive analysis of the venom gland transcriptome and proteome of <i>Cupiennius salei</i> focusses on proteins and cysteine-containing peptides and offers new insight into the structure and function of spider venom, here described as the dual prey-inactivation strategy. After venom injection, many enzymes and prot ...[more]

PMID: 30893800

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