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Venom composition does not vary greatly between different nematocyst types isolated from the primary tentacles of Olindias sambaquiensis (Cnidaria: Hydrozoa)

ABSTRACT: A quantitative proteomic study to determine the variety and relative abundance of toxins present in two nematocyst types isolated from primary tentacles of adult medusa stage Olidias sambaquiensis. Quantitative proteomics was performed with Tandem Mass Tags (TMT) to compare similarities and also differences between the two sample types using liquid chromatography coupled to tandem mass spectrometry

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Olindias Sambaquiensis

TISSUE(S): Nematocyst

SUBMITTER: Steven Lynham

LAB HEAD: Professor Paul Long

PROVIDER: PXD012887 | Pride | 2019-08-26

REPOSITORIES: Pride

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			Action	DRS
	PR461_PL_IEF10_SPS_TMT.raw	Raw
	PR461_PL_IEF11_SPS_TMT.raw	Raw
	PR461_PL_IEF12_SPS_TMT.raw	Raw
	PR461_PL_IEF1_SPS_TMT.raw	Raw
	PR461_PL_IEF2_SPS_TMT.raw	Raw

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Publications

Venom Composition Does Not Vary Greatly Between Different Nematocyst Types Isolated from the Primary Tentacles of <i>Olindias sambaquiensis</i> (Cnidaria: Hydrozoa).

Doonan Liam B LB Lynham Steven S Quinlan Catherine C Ibiji Spike C SC Winter Carlos E CE Padilla Gabriel G Jaimes-Becerra Adrian A Morandini André C AC Marques Antonio C AC Long Paul F PF

The Biological bulletin 20190807 1

In this quantitative proteomics study we determined the variety and relative abundance of toxins present in enriched preparations of two nematocyst types isolated from the primary tentacles of the adult medusa stage of the hydrozoan <i>Olindias sambaquiensis</i>. The two nematocyst types were microbasic mastigophores and microbasic euryteles, and these were recovered from the macerated tentacle tissues by using a differential centrifugation approach. Soluble protein extracts from these nematocys ...[more]

PMID: 31441701

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