Proteomics

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The Hsp70 Chaperone System Stabilizes a Thermo-sensitive Subproteome


ABSTRACT: Stress-inducible molecular chaperones have essential roles in maintaining protein homeostasis, but the extent to which they affect global proteome stability remains unclear. Here, we analyzed the effects of the DnaK (Hsp70) system on protein stability in Escherichia coli using pulse proteolysis combined with quantitative proteomics. We quantified ~1500 soluble proteins and found ~500 of them to be protease-sensitive under normal growth conditions, indicating a high prevalence of conformationally dynamic states. Acute heat stress resulted in unfolding of an additional ~200 proteins, reflected in exposure of otherwise buried hydrophobic regions. Overexpression of the DnaK chaperone system markedly stabilized most thermo-sensitive proteins, including numerous ribosomal proteins as well as large, hetero-oligomeric proteins that frequently contain the evolutionary ancient c.37 fold (P-loop nucleoside triphosphate hydrolases).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Escherichia Coli

SUBMITTER: Liang Zhao  

LAB HEAD: Liang Zhao

PROVIDER: PXD012929 | Pride | 2019-06-24

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20141005_Exactive_LZ_12671.raw Raw
20141005_Exactive_LZ_12672.raw Raw
20141005_Exactive_LZ_12673.raw Raw
20141005_Exactive_LZ_12674.raw Raw
20141005_Exactive_LZ_12675.raw Raw
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Publications

The Hsp70 Chaperone System Stabilizes a Thermo-sensitive Subproteome in E. coli.

Zhao Liang L   Vecchi Giulia G   Vendruscolo Michele M   Körner Roman R   Hayer-Hartl Manajit M   Hartl F Ulrich FU  

Cell reports 20190701 5


Stress-inducible molecular chaperones have essential roles in maintaining protein homeostasis, but the extent to which they affect overall proteome stability remains unclear. Here, we analyze the effects of the DnaK (Hsp70) system on protein stability in Escherichia coli using pulse proteolysis combined with quantitative proteomics. We quantify ∼1,500 soluble proteins and find ∼500 of these to be protease sensitive under normal growth conditions, indicating a high prevalence of conformationally  ...[more]

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