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Mapping the micro-proteome of the nuclear lamina and lamin associated domains

ABSTRACT: The nuclear lamina is a proteinaceous network of filaments that provide both structural and gene regulatory functions by tethering proteins and large domains of DNA, so-called lamin associated domains (LADs), to the periphery of the nucleus. LADs are a large fraction of the mammalian genome that are repressed, in part, by their association to the nuclear periphery. The genesis and maintenance of LADs is poorly understood as are the proteins that participate in these functions. In an effort to identify proteins that reside at the nuclear periphery and potentially interact with LADs, we have taken a two-pronged approach. First, we have undertaken an interactome analysis of the inner nuclear membrane bound LAP2β to further characterize the nuclear lamina proteome. To accomplish this, we have leveraged the BioID system, which previously has been successfully used to characterize the nuclear lamina proteome. Second, we have established a system to identify proteins that bind to LADs by developing a chromatin directed BioID system. We combined the BioID system with the m6A-tracer system which binds to LADs in live cells to identify LAD proximal and nuclear lamina proteins. In combining these datasets, we have further characterized the protein network at the nuclear lamina as well as identified putative LAD proximal proteins. Our analysis identifies many heterochromatin related proteins related to H3K9 methylation processes as well as many proteins related to cell cycle regulation identifying important proteins essential for LAD function.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Akhilesh Pandey

LAB HEAD: Akhilesh Pandey

PROVIDER: PXD012943 | Pride | 2021-03-09

REPOSITORIES: Pride

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Source:

			Action	DRS
	JC_JC9_033018_ALL_REPS-1.msf	Msf
	JC_JC9_033018_REP1.raw	Raw
	JC_JC9_033018_REP2.raw	Raw
	JC_KR1_Plus_vs_Minus_Shield.pep.xml	Pepxml
	JC_KR1_Plus_vs_Minus_Shield.prot.xml	Xml

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Mapping the micro-proteome of the nuclear lamina and lamin associated domains

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