Proteomics

Dataset Information

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Grb2 interactome data with and without EGF stimulation

ABSTRACT: Growth factor receptor-bound protein 2 or Grb2 is an adaptor protein involved in signal transduction. It is widely expressed and integral to several cellular functions, including cell proliferation. We designed this study to capture interacting partners of Grb2 in absence and presence of epidermal growth factor (EGF). HEK 293 cells, expressing tagged Grb2, were cultured with SILAC labels in presence and absence of EGF. Grb2 interactome was enriched by immunoprecipitation and subjected to mass spectrometry. This data will help in understanding the regulatory effect of EGF stimulation on Grb2 protein and its interactors.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Early Embryonic Cell

DISEASE(S): Cancer

SUBMITTER: Ajay Kumar  

LAB HEAD: Ajay Kumar

PROVIDER: PXD012957 | Pride | 2019-03-05

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
23_JUNE_2014_GRB2_HA4STRP4_IDA1.wiff Wiff
23_JUNE_2014_GRB2_HA4STRP4_IDA1.wiff.scan Wiff
23_JUNE_2014_GRB2_HA4STRP4_IDA2.wiff Wiff
23_JUNE_2014_GRB2_HA4STRP4_IDA2.wiff.scan Wiff
23_JUNE_2014_SHWETA_GRB2-IDA2_HA3STRP3_.wiff Wiff
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Publications

Outlining the Grb2 interactome data and its interacting partners in HEK293 cells in absence and presence of epidermal growth factor.

Duggal Shweta S   Midha Mukul Kumar MK   Kumar Ajay A   Rao Kanury V S KVS  

Data in brief 20190530

Growth factor receptor-bound protein 2 (Grb2) is an adaptor protein involved in the signal transduction pathways. This dataset enlists proteins which interact with Grb2 in the presence and absence of a mitogenic stimulus. Grb2 expressing HEK293 cells were cultured in light and heavy labeled SILAC media. Normal lysine and arginine were incorporated as light labels while 8 and 10 Da heavier labels of respective isotopes were used for heavy labeling. While light labeled cells were used to enrich ba  ...[more]

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