Proteomics

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Identification of Siglec-E-ligand candidates on BCL-1 mouse B cell line by proximity labeling

ABSTRACT: Siglec-E is a member of Siglec family of glycan recognition proteins involved in the regulation of myeloid cell activities. Siglec-E preferentially recognizes disialic acid, a glycan epitope consisting of two sialic acids conjugated in alpha2-8 linkage (Zhang JQ et al., Eur J Immunol. 2004, 34:1175-84). BCL-1, a mouse B cell line, expresses disialic acid epitope (Wang SH et al., Glycobiology. 2013, 23:677-89). In this experiment, we attempted at identifying the ligands of Siglec-E on BCL-1 by applying the proximity labeling method we developed (Chang L et al., J Proteome Res. 2017, 16:3929-41).

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): B Cell

SUBMITTER: Yi-Ju Chen  

LAB HEAD: Yi-Ju Chen

PROVIDER: PXD013032 | Pride | 2022-02-25

REPOSITORIES: Pride

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Action DRS
YJC-Angata_160401_BCL1mSigE_IP_InGel_01.mgf Mgf
YJC-Angata_160401_BCL1mSigE_IP_InGel_01.msf Msf
YJC-Angata_160401_BCL1mSigE_IP_InGel_01.raw Raw
YJC-Angata_160401_BCL1mSigE_IP_InGel_02.mgf Mgf
YJC-Angata_160401_BCL1mSigE_IP_InGel_02.msf Msf
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Publications

Identification of Siglec Ligands Using a Proximity Labeling Method.

Chang Lanyi L   Chen Yi-Ju YJ   Fan Chan-Yo CY   Tang Chin-Ju CJ   Chen Yi-Hsiu YH   Low Penk-Yeir PY   Ventura Albert A   Lin Chun-Cheng CC   Chen Yu-Ju YJ   Angata Takashi T  

Journal of proteome research 20170922 10

Siglecs are a family of receptor-type glycan recognition proteins (lectins) involved in self-nonself discrimination by the immune system. Identification of Siglec ligands is necessary to understand how Siglec-ligand interaction translates into biological outcomes. However, this is challenging because the interaction is weak. To facilitate identification of Siglec ligands, we adopted a proximity labeling method based on the tyramide radicalization principle. Cells that express Siglec ligands were  ...[more]

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