Dataset Information

The lactococcal phage p2 protein ORF47 impedes Lactococcus lactis MG1363 resistance to phage infection

ABSTRACT: The lactococcal phage p2 is a model for studying the Skunavirus genus, the most prevalent group of phages in cheese factories worldwide. It infects L. lactis MG1363, a model strain for the study of Gram-positive bacteria. The structural proteins of phage p2 have been thoroughly described. However, most of its non-structural proteins are still uncharacterized. Here, we developed an integrative approach, making use of structural biology, genomics, physiology, and proteomics to provide insights into the function of ORF47, the most conserved non-structural protein of unknown function among the Skunavirus genus. We found this small phage protein to have a major impact on the bacterial proteome and to be important to prevent bacterial resistance to phage infection.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Lactococcus Phage Lactococcus Lactis Subsp. Cremoris Mg1363

SUBMITTER: Sandra Maass

LAB HEAD: Doete Becher

PROVIDER: PXD013186 | Pride | 2020-07-30

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	150626_V2_PM3_CH_MLL_non_inf_t0_A_1.raw	Raw
	150626_V2_PM3_CH_MLL_non_inf_t0_A_10.raw	Raw
	150626_V2_PM3_CH_MLL_non_inf_t0_A_2.raw	Raw
	150626_V2_PM3_CH_MLL_non_inf_t0_A_3.raw	Raw
	150626_V2_PM3_CH_MLL_non_inf_t0_A_4.raw	Raw

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Publications

A Lactococcal Phage Protein Promotes Viral Propagation and Alters the Host Proteomic Response During Infection.

Lemay Marie-Laurence ML Maaß Sandra S Otto Andreas A Hamel Jérémie J Plante Pier-Luc PL Rousseau Geneviève M GM Tremblay Denise M DM Shi Rong R Corbeil Jacques J Gagné Stéphane M SM Becher Dörte D Moineau Sylvain S

Viruses 20200724 8

The lactococcal virulent phage p2 is a model for studying the <i>Skunavirus</i> genus, the most prevalent group of phages causing milk fermentation failures in cheese factories worldwide. This siphophage infects <i>Lactococcus lactis</i> MG1363, a model strain used to study Gram-positive lactic acid bacteria. The structural proteins of phage p2 have been thoroughly described, while most of its non-structural proteins remain uncharacterized. Here, we developed an integrative approach, making use ...[more]

PMID: 32722163

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