Proteomics

Dataset Information

0

Quantitative proteomic alterations of human iPSC-based neuronal development indicate early onset of Rett syndrome.


ABSTRACT: Rett syndrome (RTT) is a progressive neurodevelopmental disease often caused by mutations in the X-linked gene encoding methyl-CpG binding protein 2 (MeCP2). The mechanisms by which impaired MeCP2 induces the pathological abnormalities in the brain is not understood. To understand the molecular mechanisms involved in disease onset, we used an RTT patient induced pluripotent stem cell (iPSC)-based model and applied an in-depth high-resolution quantitative mass spectrometry-based analysis during early stages of neuronal development. Our data provided evidence of proteomic alteration at developmental stages long before the phase that symptoms of RTT syndrome become apparent. Differential expression increased from early to late neural stem cell phases, although proteins involved in immunity, metabolic processes and calcium signaling were already affected at initial stages. This data can help development of new biomarkers and therapeutic approaches in RTT syndrome.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Cell Culture

DISEASE(S): Rett Syndrome

SUBMITTER: Suzy Varderidou  

LAB HEAD: Maarten Altelaar

PROVIDER: PXD013327 | Pride | 2021-09-08

REPOSITORIES: Pride

altmetric image

Publications

Quantitative proteomic analysis of Rett iPSC-derived neuronal progenitors.

Varderidou-Minasian Suzy S   Hinz Lisa L   Hagemans Dominique D   Posthuma Danielle D   Altelaar Maarten M   Heine Vivi M VM  

Molecular autism 20200527 1


<h4>Background</h4>Rett syndrome (RTT) is a progressive neurodevelopmental disease that is characterized by abnormalities in cognitive, social, and motor skills. RTT is often caused by mutations in the X-linked gene encoding methyl-CpG binding protein 2 (MeCP2). The mechanism by which impaired MeCP2 induces the pathological abnormalities in the brain is not understood. Both patients and mouse models have shown abnormalities at molecular and cellular level before typical RTT-associated symptoms a  ...[more]

Similar Datasets

2022-10-15 | PXD033696 | Pride
2010-09-29 | E-GEOD-24329 | biostudies-arrayexpress
2022-10-14 | PXD030426 | Pride
2018-04-05 | GSE112663 | GEO
2009-12-19 | E-MEXP-1956 | biostudies-arrayexpress
2011-11-01 | E-GEOD-8720 | biostudies-arrayexpress
2021-06-25 | GSE124239 | GEO
2021-06-25 | GSE154111 | GEO
2021-06-25 | GSE124240 | GEO
2021-06-25 | GSE124238 | GEO