Dataset Information

2D Spatial Proteomic Mapping Shows Distinct Regional Differences in the Bovine Cardiac Proteome

ABSTRACT: This study highlights that organs can have variable sub-proteomes that do not necessarily correspond to anatomical features. The bovine heart as a model demonstrates at least 3-5 separate proteomes exist within a 2D plane of the heart. The main differences between these proteomes were the biochemical pathways utilized energy substrates. By mapping individual protein expression across a 2D image we can see where proteins of interest such as disease associated proteins are specifically expressed and how specific areas of the heart have subtle but significant differences in the ratio of mitochondrial complexes. This may provide an idea of where to focus analysis on in the cardiac proteome for future studies and into the different mechanisms associated with function, stresses and potential disease processes We set out to fully proteomically characterise a whole section of heart to see if the degree of proteome changes with very different anatomical and physiological function and which biochemical pathways are more prevalent or important in different areas and whether this could be explained by the physiological requirement of that area of the heart.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Heart

SUBMITTER: Wendy Heywood

LAB HEAD: Dr Kevin Mills

PROVIDER: PXD013375 | Pride | 2024-10-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	BosTaurusRefProteomeMAR2017.fasta	Fasta
	Bovine Heart map.DerivedFromSearchaf5e5381-259b-41df-b958-ffd9548290a1.pride.mgf.gz	Mgf
	Bovine Heart map.pride.mztab.gz	Mztab
	Bovine_Heart_map.DerivedFromSearchaf5e5381-259b-41df-b958-ffd9548290a1.mgf	Mgf
	Bovine_Heart_map.mzid.gz	Mzid

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Publications

A Proof of Principle 2D Spatial Proteome Mapping Analysis Reveals Distinct Regional Differences in the Cardiac Proteome.

Heywood Wendy E WE Searle Jon J Collis Richard R Doykov Ivan I Ashworth Michael M Sebire Neil N Bamber Andrew A Gautel Mathias M Eaton Simon S Coats Caroline J CJ Elliott Perry M PM Mills Kevin K

Life (Basel, Switzerland) 20240801 8

Proteomics studies often explore phenotypic differences between whole organs and systems. Within the heart, more subtle variation exists. To date, differences in the underlying proteome are only described between whole cardiac chambers. This study, using the bovine heart as a model, investigates inter-regional differences and assesses the feasibility of measuring detailed, cross-tissue variance in the cardiac proteome. Using a bovine heart, we created a two-dimensional section through a plane go ...[more]

PMID: 39202712

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