Dataset Information

Functions of the FtsH1/3 complex in the nutrient stress response of Synechocystis

ABSTRACT: FtsH zinc metalloproteinases are membrane-intrinsic and occur in bacteria, chloroplasts, and mitochondria. They are involved in diverse cellular processes including protein quality control and regulation. The cyanobacterium Synechocystis possesses four FtsH homologs, designated FtsH1-4 which function as both homo- and hetero-oligomeric complexes. For example, the FtsH1/3 complex is known to have a critical role in iron homeostasis. This project is aimed at the investigation of the involvement of the FtsH1/3 complex in cyanobacterial responses to nitrogen, phosphate and inorganic carbon starvation. By proteomic comparisons of the effects of these nutrient stresses in WT and an FtsH1-down mutant, we demonstrate significant differences in the abundances of transcription factors that complement the responses to nutrient stress at the transcriptome level. We propose a mechanism of epistatic regulation of stress-response gene expression by FtsH1/3 complex-mediated degradation of suppressor transcription factors.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Synechocystis Sp. (strain Pcc 6803 / Kazusa)

SUBMITTER: Philip Jackson

LAB HEAD: Joseph Komenda

PROVIDER: PXD013401 | Pride | 2021-08-13

REPOSITORIES: pride

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Depletion of the FtsH1/3 Proteolytic Complex Suppresses the Nutrient Stress Response in the Cyanobacterium <i>Synechocystis</i> sp strain PCC 6803.

Krynická Vendula V Georg Jens J Jackson Philip J PJ Dickman Mark J MJ Hunter C Neil CN Futschik Matthias E ME Hess Wolfgang R WR Komenda Josef J

The Plant cell 20191015 12

The membrane-embedded FtsH proteases found in bacteria, chloroplasts, and mitochondria are involved in diverse cellular processes including protein quality control and regulation. The genome of the model cyanobacterium <i>Synechocystis</i> sp PCC 6803 encodes four FtsH homologs designated FtsH1 to FtsH4. The FtsH3 homolog is present in two hetero-oligomeric complexes: FtsH2/3, which is responsible for photosystem II quality control, and the essential FtsH1/3 complex, which helps maintain Fe home ...[more]

PMID: 31615847

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