Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Caenorhabditis Elegans
TISSUE(S): Whole Body
SUBMITTER: Kathrin Theil
LAB HEAD: Nikolaus Rajewsky
PROVIDER: PXD013720 | Pride | 2019-08-12
REPOSITORIES: Pride
Items per page: 1 - 5 of 38 |
Nature communications 20190916 1
Understanding regulation of an mRNA requires knowledge of its regulators. However, methods for reliable de-novo identification of proteins binding to a particular RNA are scarce and were thus far only successfully applied to abundant noncoding RNAs in cell culture. Here, we present vIPR, an RNA-protein crosslink, RNA pulldown, and shotgun proteomics approach to identify proteins bound to selected mRNAs in C. elegans. Applying vIPR to the germline-specific transcript gld-1 led to enrichment of kn ...[more]