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Adaptation of Dinoroseobacter shibae to different kinds of oxidative stress

ABSTRACT: The Gram-negative photoheterotrophic bacterium Dinoroseobacter shibae is a member of the high abundant marine Roseobacter group. Living in the photic zone environment of marine ecosystems D. shibae is frequently exposed to oxygen. Oxic environments are hazardous and therefore effective defense mechanisms are required. In the present study, the adaptation of D. shibae to different kinds of oxidative stresses was investigated. Hydrogen peroxide, diamide and paraquat were used as agents to trigger peroxide, thiol and superoxide stress. To define and compare the peroxide, superoxide and thiol stress stimulons in D. shibae, GeLC-MS/MS based proteomic data of cytosolic and surface associated proteins were used. Furthermore, a strain deficient in the rhizobial iron regulator (RirA) was used to study the global impact of RirA on peroxide dependent protein expression.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Dinoroseobacter Shibae (strain Dfl 12)

SUBMITTER: Martin Kucklick

LAB HEAD: Susanne Engelmann

PROVIDER: PXD013791 | Pride | 2021-03-12

REPOSITORIES: Pride

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			Action	DRS
	Diamide_30_04_19_NB_Pride.xlsx	Xlsx
	Dino_shibae_genome_all_plasmids_DB_final_Ende2016.fasta	Fasta
	H2O2_30_04_19_NB_pride.xlsx	Xlsx
	K0902_140min_2014_10_28_b.raw	Raw
	K0903_140min.raw	Raw

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