Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteome of lactate grown Eubacterium limosum ATCC 8486

ABSTRACT: Eubacterium limosum ATCC 8486 makes acetate and butyrate from various substrates and is found in the human intestine. The proteome of lactate-grown Eubacterium limosum was obtained in order to identify enzymes required for growth on this substrate, in particular to identify components that are unique to growth on lactate in comparison to other substrates for acetogenesis.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Bacteria Eubacterium Limosum

SUBMITTER: Joseph Krzycki

LAB HEAD: Joseph Adrian Krzycki

PROVIDER: PXD013806 | Pride | 2019-07-25

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	31823_Sample2_JK_8F_1.raw	Raw
	31823_Sample2_JK_8F_2.raw	Raw
	31823_Sample2_JK_8F_3.raw	Raw
	31823_Sample2_JK_8F_4.raw	Raw
	31823_Sample2_JK_8F_5.raw	Raw

Items per page:

1 - 5 of 52

Similar Datasets

Proteome of L-carnitine-grown Eubacterium limosum ATCC 8486

Project description:Eubacterium limosum ATCC 8486 makes acetate and butyrate from various substrates and is found in the human intestine. The proteome of L-carnitine-grown Eubacterium limosum was obtained in order to identify enzymes required for growth on L-carnitine, in particular to identify components that are unique to growth on L-carnitine in comparison to other substrates for acetogenesis, such as lactic acid. L-carnitine and derviatives are converted to trimethylamine (TMA) by certain members of the gut microbiome, metabolism of TMA is now tied to progression of cardiovascular disease. Demethylation of carnitine is observed during growth of Eubacterium limosum on this substrate, and does not produce TMA. Carnitine demethylation by organisms like Eubacterium limosum could lessen TMA production in the gut, thereby lessening the propensity towards atherorsclerosis caused by metabolism of TMA in the body. The carnitine proteome led to the description of a carnitine:tetrahydrofolate methyltransferase system. The key carnitine demethylating enzyme is a member of the widespread TMA methyltransferase protein superfamily.

2020-06-19 | PXD013961 | Pride

Proteome of gamma-butyrobetaine-grown Eubacterium limosum ATCC 8486

Project description:Eubacterium limosum ATCC 8486 makes acetate and butyrate from various substrates and is found in the human intestine. The proteome of gamma-butyrobetaine -grown Eubacterium limosum was obtained in order to identify enzymes required for growth on gamma-butyrobetaine, in particular to identify components that are unique to growth on gamma-butyrobetaine in comparison to other substrates for acetogenesis, such as lactic acid, L-carnitine, or proline betaine. Gamma-butyrobetaine is converted to trimethylamine (TMA) by certain members of the gut microbiome. Subsequent liver metabolism of TMA is now tied to progression of cardiovascular disease. Demethylation of gamma-butyrobetaine is observed during growth of Eubacterium limosum on this substrate, and does not produce TMA. Gamma-butyrobetaine demethylation by organisms like Eubacterium limosum could lessen TMA production in the gut, thereby lessening the propensity towards atherosclerosis caused by metabolism of TMA in the body. This proteome led to discovery of gamma-butyrobetaine:tetrahydrofolate methyltransferase system. The key gamma-butyrobetaine demethylating enzyme is a member of the widespread TMA methyltransferase protein superfamily.

2021-11-04 | PXD020152 | Pride

Proteome of proline betaine-grown Eubacterium limosum ATCC 8486

Project description:Proline betaine, and to a much lesser extent, N-methyl L-proline, are found in citrus fruits and often present in the human metabolome. However, most of the N-methyl L-proline found in an animal model metabolome is produced by the microbiota, yet organisms and enzymes capable of producing N-methyl proline in the anoxic intestine have been unknown. The anaerobe Eubacterium limosum ATCC 8486 makes acetate and butyrate from various substrates and is found in the human intestine. We found that this organism demethylates proline betaine and excretes N-methyl proline during growth. The proteome of proline betaine-grown Eubacterium limosum was obtained in order to identify enzymes required for growth on proline, in particular to identify components that are unique to growth on proline in comparison to other substrates for acetogenesis, such as lactic acid. Comparison of the proteomes of the bacteria on proline betaine and lactic acid led to identification of the proteins involved in a proline betaine:tetrahydrofolate methyltransferase system which was biochemically verified. The key proline betaine demethylating enzyme is a member of the widespread TMA methyltransferase protein superfamily.

2019-07-25 | PXD013962 | Pride

RNA-seq analysis of Eubacterium limosum KIST612 across different carbon/energy/electron sources and growth phases.

Project description:To understand transcriptional regulation of Eubacterium limosum KIST612 across different carbon/energy/electron sources, RNAseq analysis was carried out over different substrate conditions (glucose, CO, H2/CO2).

2020-04-25 | GSE149269 | GEO

Project description:Eubacterium limosum 32_A2

| PRJEB16275 | ENA

Eubacterium limosum

Project description:Eubacterium limosum overview

| PRJNA52341 | ENA

Liquid C1 fermentation differential proteomic analysis of Eubacterium limosum

Project description:Some acetogenic bacteria, such as Eubacterium limosum, have the native ability to consume liquid C1 feedstocks, such as formate and methanol, as the sole substrate for growth. Due to high energy efficiency, and compatibility with existing infrastructure, this has sparked interest in the biotechnology industry. Previously, we reported limitations of this metabolism in batch fermentation. Here we undertook chemostat differential analysis to highlight key features and bottlenecks of metabolism. Our work serves as a reference dataset to advance understanding of liquid C1 metabolism in acetogens.

2023-04-13 | PXD040283 | Pride

Eubacterium limosum strain:32_a2

Project description:Eubacterium limosum 32_A2 genome sequencing

| PRJNA311425 | ENA

Eubacterium limosum strain:P2

Project description:Eubacterium limosum P2 genome sequencing

| PRJNA334929 | ENA

Eubacterium limosum strain:8486cho

Project description:Eubacterium limosum 8486cho genome sequencing

| PRJNA441676 | ENA