Dataset Information

Alterations in lipid profile of the aging kidney identified by MALDI imaging mass spectrometry

ABSTRACT: During aging, the kidney undergoes functional and physiological changes that are closely affiliated with chronic kidney disease (CKD). There is increasing evidence supporting the role of lipid or lipid-derived mediators in the pathogenesis of CKD and other aging-related diseases. To understand the role of lipids in various metabolic processes during kidney aging, we conducted MALDI imaging mass spectrometry (MALDI-IMS) analysis in kidneys harvested from young (2 months old, n=3) and old mice (24 months old, n=3). MALDI-IMS analysis showed an increase in ceramide level and a decrease in sphingomyelin (SM) and phosphatidylcholine (PC) levels in kidneys of old mice. The increased expression of cPLA2 and SMPD1 protein in aged kidney was confirmed by immunohistochemistry and western blot analysis. Our MALDI-IMS data showed the altered distribution of lipids in aged kidney as indicative of aging-related functional changes of the kidney. Combined analysis of MALDI-IMS and IHC confirmed lipidomic changes and expression levels of responsible enzymes as well as morphological changes.

INSTRUMENT(S): Bruker Daltonics flex series

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Kidney Field, Kidney

DISEASE(S): Disease Free,Chronic Kidney Disease

SUBMITTER: Joon Won Lee

LAB HEAD: Kwang Pyo Kim

PROVIDER: PXD013860 | Pride | 2019-06-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	50um_sample1_2_3_pos.ibd	Other
	50um_sample1_2_3_pos.imzML	Other
	50um_sample1_2neg.ibd	Other
	50um_sample1_2neg.imzML	Other
	50um_sample3-4_negative.jpg	Other

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Publications

Alterations in Lipid Profile of the Aging Kidney Identified by MALDI Imaging Mass Spectrometry.

Noh Sue Ah SA Kim Su-Mi SM Park Seon Hwa SH Kim Dong-Jin DJ Lee Joon Won JW Kim Yang Gyun YG Moon Ju-Young JY Lim Sung-Jig SJ Lee Sang-Ho SH Kim Kwang Pyo KP

Journal of proteome research 20190619 7

During aging, the kidney undergoes functional and physiological changes that are closely affiliated with chronic kidney disease (CKD). There is increasing evidence supporting the role of lipid or lipid-derived mediators in the pathogenesis of CKD and other aging-related diseases. To understand the role of lipids in various metabolic processes during kidney aging, we conducted matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) analysis in kidneys harvested from youn ...[more]

PMID: 31244212

Publication: 1/2

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