Dataset Information

Lysine acetylproteome profiling reveals key acetylated proteins involved in heat shock response in the sea cucumber Apostichopus japonicus

ABSTRACT: Intestine of a total of 9 idividuals was sampled. The individuals were collected from 3 groups: Control (C), Heat stress 6h (HS6h) and Heat stress 48h (HS48h).After protein extraction,trypsin digestion and enrichment of Lys-acetylated peptides, the eluted peptides was applied with LC-MS/MS detection and data analysis.Lysine acetylproteome profiling reveals key acetylated proteins involved in heat shock response in the sea cucumber Apostichopus japonicus

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Stichopus Japonicus (sea Cucumber)

TISSUE(S): Intestine

SUBMITTER: Dongxue Xu

LAB HEAD: Dongxue Xu

PROVIDER: PXD013929 | Pride | 2019-11-12

REPOSITORIES: Pride

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Publications

Lysine Acetylation is an Important Post-Translational Modification that Modulates Heat Shock Response in the Sea Cucumber Apostichopus japonicus.

Xu Dongxue D Wang Xuan X

International journal of molecular sciences 20190909 18

Heat stress (HS) is an important factor for the survival of the marine organism Apostichopus japonicus. Lysine acetylation is a pivotal post-translational modification that modulates diverse physiological processes including heat shock response (HSR). In this study, 4028 lysine acetylation sites in 1439 proteins were identified in A. japonicus by acetylproteome sequencing. A total of 13 motifs were characterized around the acetylated lysine sites. Gene Ontology analysis showed that ...[more]

PMID: 31505730

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Project description:Campylobacter, a major foodborne pathogen, is increasingly resistant to macrolide antibibotics. Previous findings suggeted that development of macrolide resistance in campylobacter requires a multi-step process, but the molecular mechanisms involved in the process are not known. In our study, multiple series of macrolide-resistant C. jejuni mutants were selected in vitro by stepwise exposure of C. jejuni NCTC11168 to increasing concentrations of erythromycin and tylosin. A set of the selected resistance were subjected to microarray and the the global transcriptional profile was analyzed. In this sery, DNA microarray was used to compare the gene expression profiles of macrolide resistant strains (68E1, 68E8 and 68E64) with its parent wild-type strain NCTC11168. The assay identified a small number of genes that showed significant changes (q-value<0.1) in expression in the low-level macrolide resistant strain 68E1, while a large number of gene showing significant changes in intermedia-level resistant stran 68E8 and high-level resistant strain 68 E64. The up-regulated genes in the resistant strains are involved in miscellaneous periplasmic proteins, efflux protienand putative aminotransferase, while the majority of the down-regulated genes are involved in electron transport,lipoprotein, heat shock protein and unknown function proteins. These findings suggest that there is not much change in low-level macrolide resistant C. jejuni strain. The over-expression of efflux pump and periplasmic protein was involved in the development of resistance to macrolide in C. jejuni. Keywords: macrolide resistant C. jejuni selected from NCTC 11168 step-wise selection. The design utilized an available two color microarray slide for the entire transcriptome of Campylobacter jejuni. Four hybridizations were performed each with independently extracted samples of either macrolide susceptible C. jejuni NCTC11168 cDNA samples or macrolide resistant C.jejuni cDNA samples. A dye swap was utilized to help minimize dye dependent bias. Thus, there were four biological replicates of each sample.

			Action	DRS
	sample1control1.raw	Raw
	sample2control2.raw	Raw
	sample3control3.raw	Raw
	sample4HS6h1.raw	Raw
	sample5HS6h2.raw	Raw

Dataset Information

Lysine acetylproteome profiling reveals key acetylated proteins involved in heat shock response in the sea cucumber Apostichopus japonicus

Dataset's files

Publications

Lysine Acetylation is an Important Post-Translational Modification that Modulates Heat Shock Response in the Sea Cucumber <i>Apostichopus japonicus</i>.

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