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Geobacter sulfurreducens and Pseudomonas aeruginosa adapted cocultures SWATH-MS

ABSTRACT: In this study we further investigate the previously observed syntrophic interaction between Geobacter sulfurreducens and Pseudomonas aeruginosa. In early coculture establishment, two genetic variants of G. sulfurreducens emerged that were strongly selected for throughout coculture evolution. Single nucleotide polymorphism (SNP) variants occurred in fabI and tetR genes of G. sulfurreducens. The tetR variants displayed upregulation of adenylate cyclase transporter, CyaE and of RND efflux pump proteins as determined through SWATH-MS proteomics.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Geobacter Sulfurreducens Pca Bacteria

SUBMITTER: Lucie Semenec

LAB HEAD: Ashley E Franks

PROVIDER: PXD013990 | Pride | 2020-04-20

REPOSITORIES: pride

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Dataset's files

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			Action	DRS
	20160719_AWMC_AndrewLaloo_IDA_41PAO1PCA.group	Other
	20160719_AWMC_AndrewLaloo_IDA_42PA14PCA.group	Other
	20160719_AWMC_AndrewLaloo_IDA_43PA14PCA.group	Other
	20160719_AWMC_AndrewLaloo_SWATH_01DC_S13.wiff	Wiff
	20160719_AWMC_AndrewLaloo_SWATH_01DC_S13.wiff.scan	Wiff

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1 - 5 of 45

Publications

Adaptive Evolution of Geobacter sulfurreducens in Coculture with Pseudomonas aeruginosa.

Semenec Lucie L Vergara Ismael A IA Laloo Andrew E AE Petrovski Steve S Bond Philip L PL Franks Ashley E AE

mBio 20200407 2

Interactions between microorganisms in mixed communities are highly complex, being either syntrophic, neutral, predatory, or competitive. Evolutionary changes can occur in the interaction dynamics between community members as they adapt to coexistence. Here, we report that the syntrophic interaction between Geobacter sulfurreducens and Pseudomonas aeruginosa coculture change in their dynamics over evolutionary time. Specifically, Geobacter sp. dominance increases with adapta ...[more]

PMID: 32265334

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